Anti-Flotillin 2/ESA抗体(ab96507)
Key features and details
- Rabbit polyclonal to Flotillin 2/ESA
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Flotillin 2/ESA抗体
参阅全部 Flotillin 2/ESA 一抗 -
描述
兔多克隆抗体to Flotillin 2/ESA -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Flotillin 2/ESA aa 365-428 (C terminal).
Database link: Q14254 -
阳性对照
- WB: HeLa. Mouse brain lysate. Rat brain lysate. IP: Human HeLa whole cell lysate. IHC-P: human lung cancer. ICC/IF: HeLa S3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96507于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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IHC-P |
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
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ICC/IF |
1/100 - 1/1000.
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IP | (1) |
1/100 - 1/500.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
IHC-P
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
靶标
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功能
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. -
组织特异性
In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. -
序列相似性
Belongs to the band 7/mec-2 family. Flotillin subfamily. -
细胞定位
Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae. - Information by UniProt
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数据库链接
- Entrez Gene: 2319 Human
- Entrez Gene: 14252 Mouse
- Entrez Gene: 83764 Rat
- Omim: 131560 Human
- SwissProt: Q14254 Human
- SwissProt: Q60634 Mouse
- SwissProt: Q9Z2S9 Rat
- Unigene: 514038 Human
see all -
别名
- ECS-1 antibody
- ECS1 antibody
- Epidermal surface antigen 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (ab96507)
Immunohistochemical analysis of paraffin-embedded human lung cancer using ab96507 at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 47 kDa -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa S3 cells, using ab96507 at 1:100 dilution. Image 2: Merged with DNA probe.
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Flotillin 2 was immunoprecipitated from 5µ of HeLa whole cell lysate with ab96507. Western blot was performed from the immunoprecipitate using ab96507. EasyBlot anti-Rabbit IgG, was used as secondary antibody.
Lane 1: HeLa lysate 5µg (Input).
Lane 2: ab96507 IP in HeLa lysate. -
Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + Rat brain lysate at 50 µg
Predicted band size: 47 kDa10% SDS-PAGE
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Immunoprecipitation - Anti-Flotillin 2/ESA antibody (ab96507)This image is courtesy of an Abreview submitted by Prof Ritva Tikkanen
Flotillin 2/ESA immunoprecipitation from Human HeLa whole cell lysate using ab96507. 750 µg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.
Lane 1 : anti-Flotillin 2/ESA IP (using non-Abcam antibody)
Lane 2 : anti-Flotillin 2/ESA IP (using ab96507)
Lane 3 : Input (HeLa cell lysate)
Lane 4 : Supernatant IP (using non-Abcam antibody)
Lane 5 : Supernatant IP (using ab96507)
Western blot detection : mouse anti-Flotillin 2/ESA antibody + HRP donkey anti-mouse polyclonal secondary antibody -
Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab96507 被引用在 6 文献中.
- Biernatowska A et al. MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules. Cells 11:N/A (2022). PubMed: 35159121
- Bai C et al. Cryopreservation disrupts lipid rafts and heat shock proteins in yellow catfish sperm. Cryobiology 87:32-39 (2019). PubMed: 30876909
- Li T et al. FLOT2 overexpression is associated with the progression and prognosis of human colorectal cancer. Oncol Lett 17:2802-2808 (2019). PubMed: 30854055
- Gong J et al. Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance. J Cell Biol 214:35-44 (2016). PubMed: 27354374
- Zhu Q et al. Overexpression of acylglycerol kinase is associated with poorer prognosis and lymph node metastasis in nasopharyngeal carcinoma. Tumour Biol 37:3349-57 (2016). WB, IHC . PubMed: 26443540
- Wang X et al. Flotillin-2 is associated with breast cancer progression and poor survival outcomes. J Transl Med 11:190 (2013). WB . PubMed: 23945257