重组Anti-Flotillin 1抗体[EP446Y] (ab78178)
![Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)](https://www.abcam.cn/ps/products/78/ab78178/Images/ab78178-395401-anti-flotillin-1-antibody-ep446y-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP446Y] to Flotillin 1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Flotillin 1抗体[EP446Y]
参阅全部 Flotillin 1 一抗 -
描述
兔单克隆抗体[EP446Y] to Flotillin 1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Flotillin 1 aa 50-150. The exact sequence is proprietary.
Database link: O75955 -
阳性对照
- WB: HEK293T, A431, HAP1, C6, K562 and HeLa cell lysates; Mouse and rat brain lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP446Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78178于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 48 kDa.
For unpurified, use 1/500 - 1/1000. |
| 说明 |
|---|
|
WB
1/2000. Predicted molecular weight: 48 kDa. For unpurified, use 1/500 - 1/1000. |
靶标
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功能
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. -
序列相似性
Belongs to the band 7/mec-2 family. Flotillin subfamily. -
细胞定位
Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 10211 Human
- Entrez Gene: 14251 Mouse
- Entrez Gene: 64665 Rat
- Omim: 606998 Human
- SwissProt: O75955 Human
- SwissProt: O08917 Mouse
- SwissProt: Q9Z1E1 Rat
- Unigene: 179986 Human
see all -
别名
- FLOT 1 antibody
- FLOT1 antibody
- FLOT1_HUMAN antibody
see all
图片
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Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : FLOT1 knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab78178 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.
ab78178 Anti-Flotillin 1 antibody [EP446Y] was shown to specifically react with Flotillin 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267276 (knockout cell lysate ab257109) was used. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab78178 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)](https://www.abcam.cn/ps/products/78/ab78178/Images/ab78178-301796-anti-flotillin-1-antibody-ep446y-western-blot.jpg)
Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Flotillin 1 knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab78178 observed at 47 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab78178 was shown to specifically react with Flotillin 1 in wild-type HAP1 cells as signal was lost in Flotillin 1 knockout cells. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab78178 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)](https://www.abcam.cn/ps/products/78/ab78178/Images/ab78178-254458-anti-flotillin-1-antibody-ep446y-western-blot.jpg)
Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution (purified)
Lane 1 : mouse brain lysate at 10 µg
Lane 2 : rat brain lysate at 20 µg
Lane 3 : C6 cell lysate at 20 µg
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 48 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)](https://www.abcam.cn/ps/products/78/ab78178/Images/ab78178-254457-anti-flotillin-1-antibody-ep446y-western-blot.jpg)
Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution (purified)
Lane 1 : K562 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 48 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)](https://www.abcam.cn/ps/products/78/ab78178/Images/ab78178-93979-anti-flotillin-1-antibody-ep446y-western-blot.jpg)
Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/500 dilution (unpurified) + K562 cell lysate
Predicted band size: 48 kDa
Observed band size: 48 kDa -
![Anti-Flotillin 1 antibody [EP446Y] (ab78178)](https://www.abcam.cn/ps/products/78/ab78178/Images/ab78178-8-benefits-of-recombinant-antibodies.png)
Anti-Flotillin 1 antibody [EP446Y] (ab78178)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab78178 被引用在 1 文献中.
- Valdivia AO & Bhattacharya SK Lyso-Lipid-Induced Oligodendrocyte Maturation Underlies Restoration of Optic Nerve Function. eNeuro 9:N/A (2022). PubMed: 35027445