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Anti-FLK1抗体[Clone 10] (ab24313)

Reviews (1)Q&A (1)References (1)
Western blot - Anti-FLK1 antibody [Clone 10] (ab24313)

    Key features and details

    • Mouse monoclonal [Clone 10] to FLK1
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG

    概述

    • 产品名称

      Anti-FLK1抗体[Clone 10]

    • 描述

      小鼠单克隆抗体[Clone 10] to FLK1

    • 宿主

      Mouse

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Saccharomyces cerevisiae

    • 免疫原

      Recombinant fragment corresponding to Saccharomyces cerevisiae FLK1 aa 1-200.

    • 阳性对照

      • Mav108 cells expressing recombinant FLK1.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab24313于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 78 kDa).
    说明
    WB
    Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 78 kDa).

    靶标

    • 相关性

      Yeast TUP1 Ssn6p repressor complex represents a novel paradigm for transcriptional repression and for the role of chromatin in repression. TUP1 and SSN6 are involved in repression of several diverse families of genes in yeast, including cell type specific genes regulated by the alpha2 and a1/alpha2 repressors. Ssn6 Tupl interacts with class I histone deacetylases required for repression, and recruitment of yeast Tuplp Ssn6p repressor is associated with localised decreases in histone acetylation.

    • 细胞定位

      Nuclear

    • 数据库链接

      • 别名

        • FLK1 antibody
        • Flocculation suppressor protein antibody
        • Repressor AER2 antibody
        • SFL2 antibody
        • Tup1p antibody
        • UMR7 antibody
        see all

      图片

      • Western blot - Anti-FLK1 antibody [Clone 10] (ab24313)
        Western blot - Anti-FLK1 antibody [Clone 10] (ab24313)Image is courtesy of an anonymous AbReview.
        All lanes : Anti-FLK1 antibody [Clone 10] (ab24313)

        All lanes : Saccharomyces cerevisiae whole cell lysates

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Sheep anti-Mouse polyclonal HRP conjugate at 100 %

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 78 kDa
        Observed band size: 90 kDa why is the actual band size different from the predicted?


        Exposure time: 1 minute

        See Abreview

      文献 (1)

      发表研究结果有使用 ab24313？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab24313 被引用在 1 文献中.

      • Mei B  et al. The regulatory mechanism and biological significance of the Snail-miR590-VEGFR-NRP1 axis in the angiogenesis, growth and metastasis of gastric cancer. Cell Death Dis 11:241 (2020). PubMed: 32303680

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-TUP1 antibody [Clone 10]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Saccharomyces cerevisiae Cell lysate - whole cell (whole cell)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      15 µg
      Specification
      whole cell
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Oct 30 2015

      Question

      Why is the detected band size 35kDa but the predicted band size 78kDa? Some journals also mentinoed that the detected band size is around 90kDa.

      Read More
      Answer

      Thank you for your enquiry. The cell line used was transfected to express a fragment of the TUP1 protein, which was the antigen used to develop the antibody. This protein fragment has a molecular weight of approximately 35 kDa. Therefore, the 35kDa band was detected in WB. Unfortunately, we have not tested the product using full length protein and therefore, it is possible that the higher band size you observed with yuo r samples could be the full length protein.

      Read More

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