AB108594

重组Anti-Flightless I抗体[EPR4201]

Anti-Flightless I antibody [EPR4201]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal Flightless I antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

查看别名

FLIL, FLII, Protein flightless-1 homolog

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] (AB108594)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] (AB108594)

ab108594 at 1/100 dilution staining Flightless I in HeLa cells by Immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] (AB108594)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] (AB108594)

ab108594 at 1/100 dilution staining Flightless I in Human skeletal muscle by Immunohistochemistry, Paraffin-embedded tissue

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-Flightless I antibody [EPR4201] (AB108594)

Lanes 1-3 : Merged signal (red and green). Green - ab108594 observed at 145 kDa. Red - loading control ab7291 observed at 50 kDa.

ab108594 Anti-Flightless I antibody [EPR4201] was shown to specifically react with Flightless I in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265890 (knockout cell lysate ab257954) was used. Wild-type and Flightless I knockout samples were subjected to SDS-PAGE. ab108594 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Flightless I antibody [EPR4201] (ab108594) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

FLII knockout HeLa cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 145 kDa

Observed band size: 145 kDa

false

Unknown

Western blot - Anti-Flightless I antibody [EPR4201] (AB108594)

All lanes:

Western blot - Anti-Flightless I antibody [EPR4201] (ab108594) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Predicted band size: 145 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Flightless I antibody [EPR4201] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4201

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Antigen retrieval is recommended.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Flightless I also known as FLII is a multifunctional protein with a mass of approximately 131 kDa. This protein belongs to the gelsolin family and contains leucine-rich repeats (LRR) and a partially functional gelsolin domain. It is widely expressed in various tissues including muscle and skin. Functionally Flightless I interacts with actin filaments and plays a role in modulating the actin cytoskeleton. Through its actin-binding property Flightless I participates in cellular processes such as motility and structural maintenance.

Biological function summary

Flightless I protein influences several cell mechanisms by associating with signaling complexes. It functions as a part of cellular assemblies where it modulates actin dynamics and affects transcriptional activities by interacting with other cellular components. Additionally Flightless I contributes to cell growth survival and wound healing processes. The protein can also regulate certain gene expressions by acting as a transcriptional co-regulator highlighting its involvement in cellular communication.

Pathways

Flightless I has significant roles in cellular signaling and cytoskeletal networks. It is actively involved in the Wnt signaling pathway as well as the Toll-like receptor signaling pathway. These pathways are central to cell communication and immune response regulation. Flightless I operates alongside proteins such as Dishevelled and MyD88 integrating signals that influence cell behavior and immune reactions. By mediating these interactions Flightless I enhances the cellular response to environmental cues.

Flightless I is associated with aberrant conditions like cancer and muscular dystrophy. Its dysregulation has been linked to tumor progression and metastasis where it influences cell migration and invasion. Flightless I interacts with proteins such as beta-catenin and dystrophin in these contexts affecting cellular adhesion and stability. Understanding these interactions can provide insights into therapeutic targeting strategies for managing and treating associated pathologies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Is a regulator of actin polymerization, required for proper myofibril organization and regulation of the length of sarcomeric thin filaments (By similarity). It also plays a role in the assembly of cardiomyocyte cell adhesion complexes (By similarity). Regulates cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation (By similarity). May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1 (PubMed : 14966289). Involved in estrogen hormone signaling.

See full target information FLII

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Basic & clinical pharmacology & toxicology 127:389-404 PubMed32524749

2020

Nucleoredoxin interaction with flightless-I/actin complex is differentially altered in alcoholic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Brisa Rodope Alarcón-Sánchez,Dafne Guerrero-Escalera,Sandra Rosas-Madrigal,Diana Ivette Aparicio-Bautista,Karina Reyes-Gordillo,M Raj Lakshman,Arturo Ortiz-Fernández,Héctor Quezada,Óscar Medina-Contreras,Saúl Villa-Treviño,Julio Isael Pérez-Carreón,Jaime Arellanes-Robledo

Molecular biology of the cell 26:2279-97 PubMed25877872

2015

Flightless I interacts with NMMIIA to promote cell extension formation, which enables collagen remodeling.

Applications

Species

Unspecified reactive species

Pamma D Arora,Yongqiang Wang,Anne Bresnick,Paul A Janmey,Christopher A McCulloch

View all publications

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重组Anti-Flightless I抗体[EPR4201]