Anti-FLI1抗体- C-terminal (ab180902)
Key features and details
- Rabbit polyclonal to FLI1 - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-FLI1抗体- C-terminal
参阅全部 FLI1 一抗 -
描述
兔多克隆抗体to FLI1 - C-terminal -
宿主
Rabbit -
特异性
At least four isoforms of FLI1 are known to exist; this antibody will detect all four. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human FLI1 (C terminal). The exact sequence is proprietary. 17 amino acid peptide near the C-terminus of Human FLI1.
Database link: Q01543 -
阳性对照
- Jurkat whole cell lysate (ab7899); HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180902于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use a concentration of 5 µg/ml.
|
|
WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).
|
说明 |
---|
ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa). |
靶标
-
功能
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. -
疾病相关
Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. -
序列相似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 2313 Human
- Omim: 193067 Human
- SwissProt: Q01543 Human
- Unigene: 504281 Human
-
别名
- ERGB transcription factor antibody
- Ewing Sarcoma breakpoint region 2 antibody
- EWSR 2 antibody
see all
图片
-
Lane 1 : Anti-FLI1 antibody - C-terminal (ab180902) at 0.5 µg/ml
Lane 2 : Anti-FLI1 antibody - C-terminal (ab180902) at 1 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 51 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescent analysis of HeLa cells labeling FLI1 with ab180902 at 5 µg/ml.
实验方案
数据表及文件
-
Datasheet download
文献 (1)
ab180902 被引用在 1 文献中.
- Su S et al. SPOP and OTUD7A Control EWS-FLI1 Protein Stability to Govern Ewing Sarcoma Growth. Adv Sci (Weinh) 8:e2004846 (2021). PubMed: 34060252