Anti-FLASH抗体(ab8420)
Key features and details
- Rabbit polyclonal to FLASH
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FLASH抗体 -
描述
兔多克隆抗体to FLASH -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide:
SERFQQLMKLFEKSKC
, corresponding to amino acids 1966/1981 of Human FLASH. (Peptide available as ab8455.) -
阳性对照
- HeLa cell lysate An approximate 220 kDa band can be detected.
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常规说明
A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Affinity purified -
Primary antibody说明
A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2). -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Human FLASH peptide (ab8455).
|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Human FLASH peptide (ab8455). |
|
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus. Localizes to discrete nuclear foci. - Information by UniProt
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数据库链接
- Entrez Gene: 9994 Human
- Entrez Gene: 26885 Mouse
- Omim: 606880 Human
- SwissProt: Q9UKL3 Human
- SwissProt: Q9WUF3 Mouse
- Unigene: 558218 Human
- Unigene: 22279 Mouse
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别名
- AA387232 antibody
- C8AP2_HUMAN antibody
- CASP 8 associated protein 2 antibody
see all
图片
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Western blot - Anti-FLASH antibody (ab8420)Anti-FLASH antibody (ab8420) at 0.5 µg/ml + HeLa whole cell lysate
Observed band size: 220 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-FLASH antibody (ab8420)ab8420 at 10µg/ml staining FLASH in Hela cells by ICC/IF
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab8420 被引用在 2 文献中.
- Gajdušková P et al. CDK11 is required for transcription of replication-dependent histone genes. Nat Struct Mol Biol 27:500-510 (2020). PubMed: 32367068
- Barcaroli D et al. FLASH is an essential component of Cajal bodies. Proc Natl Acad Sci U S A : (2006). PubMed: 17003126