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AB8420

Anti-FLASH抗体

Anti-FLASH antibody

3

(2 Reviews)

|

(3 Publications)

Rabbit Polyclonal FLASH antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CASP8AP2 aa 1950 to C-terminus.

查看别名

FLASH, KIAA1315, RIP25, CASP8AP2, CASP8-associated protein 2, FLICE-associated huge protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FLASH antibody (AB8420)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FLASH antibody (AB8420)

ab8420 at 10μg/ml staining FLASH in Hela cells by ICC/IF

Western blot - Anti-FLASH antibody (AB8420)
  • WB

Unknown

Western blot - Anti-FLASH antibody (AB8420)

All lanes:

Western blot - Anti-FLASH antibody (ab8420) at 0.5 µg/mL

All lanes:

HeLa whole cell lysate

Predicted band size: 223 kDa

Observed band size: 220 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

轻链类型

unknown

不含载体蛋白

No

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human CASP8AP2 aa 1950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UKL3

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搭配使用产品

Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB307837

Anti-NPAT antibody [27/NPAT]

Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-NPAT antibody [27/NPAT] (AB307837)

  • 0
  • 0
Primary Antibodies

AB181972

Anti-SLBP antibody [EPR12673]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SLBP antibody [EPR12673] (AB181972)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-0.5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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产品详情

A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

FLASH also known as "CASP8 and FADD-like apoptosis regulator" is an important protein that plays a role in apoptosis. This protein has a molecular mass of approximately 220 kDa and can be found expressed in various tissues including the liver kidney and heart. FLASH localizes to the nucleus where it interacts with several nuclear components and contributes to critical cellular functions. Researchers recognize its significance in regulating apoptotic pathways making it a target for studies involving cell death.
Biological function summary

The CASP8 and FADD-like apoptosis regulator is part of the complex involved in the regulation of apoptosis and cell cycle. FLASH plays its part by interacting with other apoptotic factors ensuring proper cell cycle progression and programmed cell death. It associates with the death effector domain (DED) proteins and has shown involvement in the activation of caspase-8. This regulation is significant for maintaining cellular homeostasis and preventing the development of cancerous cells.

Pathways

Studies show that FLASH integrates into the apoptotic pathway and NF-kB signaling cascade. It engages in critical interactions with proteins such as FADD and caspase-8 which are essential for the initiation and execution of apoptosis. By influencing NF-kB signaling FLASH modulates inflammatory responses and survival signals effectively balancing cell death and survival mechanisms.

Research frequently connects FLASH to cancer and autoimmune diseases. Its dysregulation can lead to aberrant apoptotic signaling contributing to cancer progression or inappropriate cell death as seen in autoimmune conditions. FLASH’s interaction with proteins like caspase-8 makes it an attractive target for developing therapies for diseases where apoptosis is disrupted. Understanding FLASH’s function and interactions provides insight into potential therapeutic strategies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Participates in TNF-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B (PubMed : 12477726, PubMed : 15698540). Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation (PubMed : 12477726, PubMed : 15698540). Involved in TNF-induced activation of NF-kappa-B via a TRAF2-dependent pathway (By similarity). Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B (PubMed : 17245429). Required for the activation of CASP8 in FAS-mediated apoptosis (PubMed : 17245429). Involved for histone gene transcription and progression through S phase : required for the generation of mature histone mRNAs following the 3' end cleavage of histone pre-mRNAs (PubMed : 17003125, PubMed : 19854135, PubMed : 32367068).
See full target information CASP8AP2
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 85:2487-2502.e12 PubMed40516529

2025

CRAMP1-dependent histone H1 biogenesis is essential for topoisomerase II inhibitor tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Ingham,Ignacio Alonso de Vega,Louise Morlot,William Gittens,Ivo A Hendriks,Ellen S Kakulidis,Raimundo Freire,Norman E Davey,Julien P Duxin,Michael Lund Nielsen,Niels Mailand

Nature structural & molecular biology 27:500-510 PubMed32367068

2020

CDK11 is required for transcription of replication-dependent histone genes.

Applications

Unspecified application

Species

Unspecified reactive species

Pavla Gajdušková,Igor Ruiz de Los Mozos,Michal Rájecký,Milan Hluchý,Jernej Ule,Dalibor Blazek

Proceedings of the National Academy of Sciences of 103:14802-7 PubMed17003126

2006

FLASH is an essential component of Cajal bodies.

Applications

Unspecified application

Species

Unspecified reactive species

D Barcaroli,D Dinsdale,M H Neale,L Bongiorno-Borbone,M Ranalli,E Munarriz,A E Sayan,J M McWilliam,T M Smith,E Fava,R A Knight,G Melino,V De Laurenzi
View all publications
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