重组Anti-FKBP52抗体[EPR6619] (ab124906)
![Western blot - Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.cn/ps/products/124/ab124906/Images/ab124906-138889-anti-fkbp52-antibody-epr6619-western-blot.jpg "Western blot - Anti-FKBP52 antibody [EPR6619] (ab124906)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6619] to FKBP52
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FKBP52抗体[EPR6619]
参阅全部 FKBP52 一抗 -
描述
兔单克隆抗体[EPR6619] to FKBP52 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide within Human FKBP52 aa 400-500 (C terminal). The exact sequence is proprietary.
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阳性对照
- HeLa, MCF7, Jurkat and K562 cell lysates; Human breast tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.38 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6619 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124906于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/50000 - 1/200000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/50000 - 1/200000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt (Intra) or ICC/IF.
靶标
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功能
Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. -
组织特异性
Widely expressed. -
序列相似性
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
结构域
The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400. -
翻译后修饰
Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 2288 Human
- Omim: 600611 Human
- SwissProt: Q02790 Human
- Unigene: 524183 Human
- Unigene: 713721 Human
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别名
- 51 kDa FK506-binding protein antibody
- 52 kDa FK506 binding protein antibody
- 52 kDa FK506-binding protein antibody
see all
图片
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Western blot - Anti-FKBP52 antibody [EPR6619] (ab124906)All lanes : Anti-FKBP52 antibody [EPR6619] (ab124906) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.cn/ps/products/124/ab124906/Images/ab124906-138888-anti-fkbp52-antibody-epr6619-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6619] (ab124906)ab124906, at 1/100 dilution, staining FKBP52 in paraffin-embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.cn/ps/products/124/ab124906/Images/ab124906-138887-anti-fkbp52-antibody-epr6619.jpg)
OI-RD Scanning - Anti-FKBP52 antibody [EPR6619] (ab124906) -
![Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.cn/ps/products/124/ab124906/Images/ab124906-4-benefits-of-recombinant-antibodies.png)
Anti-FKBP52 antibody [EPR6619] (ab124906)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab124906 被引用在 1 文献中.
- Maeda K et al. FKBP51 and FKBP52 regulate androgen receptor dimerization and proliferation in prostate cancer cells. Mol Oncol 16:940-956 (2022). PubMed: 34057812