重组Anti-FKBP52抗体[EPR6618] (ab129097)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6618] to FKBP52
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FKBP52抗体[EPR6618]
参阅全部 FKBP52 一抗 -
描述
兔单克隆抗体[EPR6618] to FKBP52 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human FKBP52 aa 1-100. The exact sequence is proprietary.
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阳性对照
- HeLa, Jurkat, K562, and MCF7 cell lysates; Human colon and kidney tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 9.40 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR6618 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129097于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
靶标
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功能
Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. -
组织特异性
Widely expressed. -
序列相似性
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
结构域
The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400. -
翻译后修饰
Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 2288 Human
- Entrez Gene: 14228 Mouse
- Entrez Gene: 260321 Rat
- Omim: 600611 Human
- SwissProt: Q02790 Human
- SwissProt: P30416 Mouse
- SwissProt: Q9QVC8 Rat
- Unigene: 524183 Human
see all -
别名
- 51 kDa FK506-binding protein antibody
- 52 kDa FK506 binding protein antibody
- 52 kDa FK506-binding protein antibody
see all
图片
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All lanes : Anti-FKBP52 antibody [EPR6618] (ab129097) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : K562 cell lysates
Lane 4 : MCF7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa -
ab129097 staining FKBP52 in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.
DAPI was used as a nuclear counterstain and the negative control was PBS only.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)
ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)
ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab129097 被引用在 11 文献中.
- Han PZ et al. Development and validation of a gene model predicting lymph node metastasis and prognosis of oral squamous cell carcinoma based on single-cell and bulk RNA-seq analysis. J Oral Pathol Med 52:389-401 (2023). PubMed: 36153671
- Varastehmoradi B et al. Kappa opioid activation changes protein profiles in different regions of the brain relevant to depression. Eur Neuropsychopharmacol 72:9-17 (2023). PubMed: 37040689
- Lin CC et al. Effects of RU486 in Treatment of Traumatic Stress-Induced Glucocorticoid Dysregulation and Fear-Related Abnormalities: Early versus Late Intervention. Int J Mol Sci 23:N/A (2022). PubMed: 35628305
- Zong S et al. FKBP4 integrates FKBP4/Hsp90/IKK with FKBP4/Hsp70/RelA complex to promote lung adenocarcinoma progression via IKK/NF-κB signaling. Cell Death Dis 12:602 (2021). PubMed: 34112753
- Shao J et al. Hyperglycemia-induced increasing of RELB/circ_0008590 in NF-κB pathway is repressed by miR-1243 in human retinal microvascular endothelial cells. Ann Transl Med 9:1624 (2021). PubMed: 34926668