Anti-FKBP51抗体(ab2901)
Key features and details
- Rabbit polyclonal to FKBP51
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FKBP51抗体
参阅全部 FKBP51 一抗 -
描述
兔多克隆抗体to FKBP51 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
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阳性对照
- WB: K562, HepG2, PC3, HeLa and MCF7 cell lysates; PC-12 and L6 cell lysates; Rat liver, brain, testis and kidney tissue lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa. |
靶标
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功能
Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. -
组织特异性
Widely expressed, enriched in testis compared to other tissues. -
序列相似性
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2289 Human
- Entrez Gene: 361810 Rat
- Omim: 602623 Human
- SwissProt: Q13451 Human
- Unigene: 407190 Human
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别名
- 51 kDa FK506 binding protein 5 antibody
- 51 kDa FK506 binding protein antibody
- 51 kDa FK506-binding protein antibody
see all
图片
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All lanes : Anti-FKBP51 antibody (ab2901) at 2 µg/ml
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) nuclear enriched extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) nuclear enriched extract
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) nuclear enriched extract
Lane 4 : MCF7 (human breast adenocarcinoma cell line) nuclear enriched extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) at 1/2500 dilutionApproximately 51 and 45 kDa bands corresponding to FKBP51 were observed across cell lines tested
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All lanes : Anti-FKBP51 antibody (ab2901) at 1 µg/ml
Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : L6 (Rat skeletal muscle cell line) whole cell lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat testis tissue lysate
Lane 6 : Rat kidney tissue lysate
Lysates/proteins at 30 µg per lane.Western blot was performed using ab2901 and a 51kDa band corresponding to FKBP5 was observed across all the tested cell lines and tissues. Whole cell extracts were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel . Resolved proteins were then transferred onto a Nitrocellulose membrane by iBlot® 2 Dry Blotting System. The blot was probed with the primary antibody (1µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP using the iBright FL 1000. Chemiluminescent detection was performed using Novex® ECL Reagent Kit.
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Anti-FKBP51 antibody (ab2901) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab2901 被引用在 18 文献中.
- Gao Z et al. FK506-binding protein 5 promotes the progression of papillary thyroid carcinoma. J Int Med Res 49:3000605211008325 (2021). PubMed: 33906532
- Corbin JM et al. Seed-mediated RNA interference of androgen signaling and survival networks induces cell death in prostate cancer cells. Mol Ther Nucleic Acids 24:337-351 (2021). PubMed: 33850637
- Zhang C et al. The deficiency of FKBP-5 inhibited hepatocellular progression by increasing the infiltration of distinct immune cells and inhibiting obesity-associated gut microbial metabolite. J Gastrointest Oncol 12:711-721 (2021). PubMed: 34012660
- Lu A et al. Heterogenetic parabiosis between healthy and dystrophic mice improve the histopathology in muscular dystrophy. Sci Rep 10:7075 (2020). PubMed: 32341395
- Hadamitzky M et al. Repeated Systemic Treatment with Rapamycin Affects Behavior and Amygdala Protein Expression in Rats. Int J Neuropsychopharmacol 21:592-602 (2018). PubMed: 29462337