Anti-FKBP15抗体(ab14432)
Key features and details
- Rabbit polyclonal to FKBP15
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-FKBP15抗体 -
描述
兔多克隆抗体to FKBP15 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human FKBP135 aa 50-150.
Database link: Q5T1M5 -
阳性对照
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antibody was purified using an epitope specific to FKBP-15 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14432于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 135 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 135 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
May be involved in the cytoskeletal organization of neuronal growth cones. Seems to be inactive as a PPIase (By similarity). Involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement. -
序列相似性
Belongs to the FKBP-type PPIase family.
Contains 1 PPIase FKBP-type domain. -
结构域
The PPIase FKBP-type domain seems to be inactive both for FK506-binding and enzymatic activity.
The central coiled-coil region is responsible for association with early endosomes. -
细胞定位
Cytoplasm. Cell projection, axon. Early endosome. Present in axons and neuronal growth cones. - Information by UniProt
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数据库链接
- Entrez Gene: 23307 Human
- SwissProt: Q5T1M5 Human
- Unigene: 522351 Human
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别名
- 133 kDa FK506-binding protein antibody
- 133 kDa FKBP antibody
- FK506-binding protein 15 antibody
see all
图片
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Western blot - Anti-FKBP135 antibody (ab14432)All lanes : Anti-FKBP15 antibody (ab14432) at 0.1 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 135 kDa
Exposure time: 3 minutes -
Western blot - Anti-FKBP135 antibody (ab14432)All lanes : Anti-FKBP15 antibody (ab14432) at 0.2 µg/ml
Lane 1 : Nuclear lysates prepared from Hela cells at 5 µg
Lane 2 : Nuclear lysates prepared from Hela cells at 20 µg
Lanes 3-5 : Nuclear lysates prepared from Hela cells at 1 mg/ml
Predicted band size: 135 kDaAntibodies: ab14432 used at 0.2 mcg/ml for WB in A and B and 2 mcg/mg extract for IP (B), ab14433 and ab14434 used at 2 mcg/mg extract for IP. Detection: Chemiluminescence with an exposure time of 25 minutes (A) and 10 minutes (B).
In figure B the Immunoprecipitation was done using ab14432 (Lane 3), ab14433 (Lane 4) and ab14434 (Lane 5) at 2µg/mg of extract.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab14432 被引用在 4 文献中.
- Zheng L et al. M6A Demethylase Inhibits Osteogenesis of Dental Follicle Stem Cells via Regulating miR-7974/FKBP15 Pathway. Int J Mol Sci 24:N/A (2023). PubMed: 38003310
- Tyrrell BJ et al. Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. Pigment Cell Melanoma Res 29:559-71 (2016). PubMed: 27390154
- Zavodszky E et al. Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy. Nat Commun 5:3828 (2014). ICC/IF ; Human . PubMed: 24819384
- Viklund IM et al. Identification of a new WASP and FKBP-like (WAFL) protein in inflammatory bowel disease: a potential marker gene for ulcerative colitis. Int J Colorectal Dis 23:921-30 (2008). IHC (PFA fixed) ; Human . PubMed: 18654788