FITC荧光Anti-Vimentin抗体[RV202] (ab128507)
Key features and details
- FITC Mouse monoclonal [RV202] to Vimentin
- Suitable for: IHC-Fr, Flow Cyt (Intra)
- Reacts with: Human, Pig
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-Vimentin抗体[RV202]
参阅全部 Vimentin 一抗 -
描述
FITC荧光小鼠单克隆抗体[RV202] to Vimentin -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: IHC-Fr, Flow Cyt (Intra)more details -
种属反应性
与反应: Human, Pig
不与反应: Zebrafish -
免疫原
Full length native protein (purified) corresponding to Cow Vimentin. Cytoskeletal vimentin extract of calf lens.
Database link: P48616 -
阳性对照
- IHC-Fr: Swine colon tissue. Flow Cyt (intra): HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
RV202 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128507于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
1/10 - 1/200.
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Flow Cyt (Intra) |
1/1000.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-Fr
1/10 - 1/200. |
Flow Cyt (Intra)
1/1000. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
组织特异性
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
疾病相关
Cataract 30 -
序列相似性
Belongs to the intermediate filament family. -
结构域
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
翻译后修饰
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 7431 Human
- Entrez Gene: 100522394 Pig
- Omim: 193060 Human
- SwissProt: P08670 Human
- SwissProt: P02543 Pig
- Unigene: 455493 Human
- Unigene: 691131 Human
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形式
Vimentin is found in connective tissue and in the cytoskeleton. -
别名
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
图片
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ab128507, at 1/200 dilution, staining Vimentin in a frozen section of Swine colon tissue by Immunohistochemistry.
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Overlay histogram showing HeLa cells stained with ab128507 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128507, 1/1000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [MOPC-21] (FITC) (ab18435, 1μg/1x106 cells) for 30 min at 22°C. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab128507 被引用在 11 文献中.
- Ye B et al. Induction of functional neutrophils from mouse fibroblasts by thymidine through enhancement of Tet3 activity. Cell Mol Immunol 19:619-633 (2022). PubMed: 35301470
- Pan P et al. Vimentin regulation of autophagy activation in lung fibroblasts in response to lipopolysaccharide exposure in vitro. Ann Transl Med 9:304 (2021). PubMed: 33708931
- Niu R et al. Follistatin-like protein 1 functions as a potential target of gene therapy in proliferative diabetic retinopathy. Aging (Albany NY) 13:8643-8664 (2021). PubMed: 33714952
- Nobakht Lahrood F et al. Generation of Transplantable Three-Dimensional Hepatic-Patch to Improve the Functionality of Hepatic Cells In Vitro and In Vivo. Stem Cells Dev 29:301-313 (2020). PubMed: 31856676
- Chen L et al. Zebrafish Microenvironment Elevates EMT and CSC-Like Phenotype of Engrafted Prostate Cancer Cells. Cells 9:N/A (2020). PubMed: 32225005