AB58864

FITC荧光Anti-TLR9抗体[5G5]

Name: FITC Anti-TLR9抗体[5G5] (ab58864)| Abcam中文官网
Brand: Abcam Limited
SKU: AB58864
Price: 1356 CNY
Availability: InStock

FITC Anti-TLR9 antibody [5G5]

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(7 Publications)

Mouse Monoclonal TLR9 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TLR9.

查看别名

CD289, UNQ5798/PRO19605, TLR9, Toll-like receptor 9

1 Images

Flow Cytometry (Intracellular) - FITC Anti-TLR9 antibody [5G5] (AB58864)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-TLR9 antibody [5G5] (AB58864)

THP1 cells were incubated with an IgG2a isotype control (A) or a-TLR9 5G5 mAb (B). Cells (140000) were permeabilized with saponin and stained with 0.4 µg 5G5.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

5G5

亚型

IgG2a

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra)

applications

免疫原

Recombinant Fragment Protein within Human TLR9. The exact immunogen used to generate this antibody is proprietary information.

Q9NR96

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/50", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/fitc-mouse-igg2a-x5563-isotype-control-ab91362'>ab91362</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

纯化说明

0.2 µm filtered

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TLR9 also known as Toll-like receptor 9 is a protein that detects unmethylated CpG motifs which are common in bacterial and viral DNA. This protein weighs about 116 kDa. It mainly expresses in plasmacytoid dendritic cells B cells and in epithelial cells of various tissues. TLR9 locates in the endoplasmic reticulum and translocates to endolysosomes upon activation which is essential for its detection function.

Biological function summary

TLR9 recognizes and binds to microbial DNA triggering an immune response. It is part of the Toll-like receptor family which plays an important role in the innate immune system. TLR9 engages in the formation of complexes with other molecules such as MyD88 an adaptor protein to propagate downstream signaling. This leads to the activation of transcription factors that stimulate cytokine production promoting inflammation and defense against infections.

Pathways

TLR9 participates significantly in the NF-kB activation pathway and the type I interferon signaling cascade. During these processes TLR9 interacts with proteins like IRAK4 and TRAF6. In the NF-kB pathway TLR9's role in the early immune response is coordinated with other Toll-like receptors to detect pathogen-related patterns and initiate adaptive immunity. Its influence on type I interferon signaling facilitates antiviral defense mechanisms.

TLR9's malfunction or altered expression associates with systemic lupus erythematosus (SLE) and cancer. In SLE overactivation of TLR9 leads to autoantibody production which worsens the disease. This can involve the protein BAFF which supports B cell proliferation. In cancer TLR9 can influence tumor growth and the tumor microenvironment affecting disease progression. Its modulation is being researched for therapeutic interventions in these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PubMed : 14716310). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 11564765, PubMed : 17932028). Controls lymphocyte response to Helicobacter infection (By similarity). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (PubMed : 23857366).

See full target information TLR9

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:954984 PubMed36591257

2022

Comparison of different gene addition strategies to modify placental derived-mesenchymal stromal cells to produce FVIII.

Applications

Unspecified application

Species

Unspecified reactive species

Ritu M Ramamurthy,Martin Rodriguez,Hannah C Ainsworth,Jordan Shields,Diane Meares,Colin Bishop,Andrew Farland,Carl D Langefeld,Anthony Atala,Christopher B Doering,H Trent Spencer,Christopher D Porada,Graça Almeida-Porada

Scientific reports 11:222 PubMed33420306

2021

Platelet CXCL4 mediates neutrophil extracellular traps formation in ANCA-associated vasculitis.

Applications

Unspecified application

Species

Unspecified reactive species

Kotaro Matsumoto,Hidekata Yasuoka,Keiko Yoshimoto,Katsuya Suzuki,Tsutomu Takeuchi

Journal of the Egyptian National Cancer Institute 32:15 PubMed32372371

2020

Toll-like receptor 9 negatively related to clinical outcome of AML patients.

Applications

Unspecified application

Species

Unspecified reactive species

Waiel M A Al-Kahiry,Enas A M Dammag,Hadeel S T Abdelsalam,Hayat K Fadlallah,Mona S Owais

PloS one 13:e0203557 PubMed30256831

2018

Tunable activation of therapeutic platelet-rich plasma by pulse electric field: Differential effects on clot formation, growth factor release, and platelet morphology.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew L Frelinger,Anja J Gerrits,V Bogdan Neculaes,Thomas Gremmel,Andrew S Torres,Anthony Caiafa,Sabrina L Carmichael,Alan D Michelson

Journal of immunology (Baltimore, Md. : 1950) 197:1905-13 PubMed27474073

2016

DNA Sensing via TLR-9 Constitutes a Major Innate Immunity Pathway Activated during Erythema Nodosum Leprosum.

Applications

Unspecified application

Species

Unspecified reactive species

André A Dias,Camila O Silva,João Pedro S Santos,Leonardo R Batista-Silva,Chyntia Carolina D Acosta,Amanda N B Fontes,Roberta O Pinheiro,Flávio A Lara,Alice M Machado,José Augusto C Nery,Euzenir N Sarno,Geraldo M B Pereira,Maria Cristina V Pessolani

Molecular therapy. Methods & clinical development 2014: PubMed25279386

2014

Evaluation of gene delivery strategies to efficiently overexpress functional HLA-G on human bone marrow stromal cells.

Applications

Flow Cyt

Species

Human

Joana S Boura,Melisa Vance,Weihong Yin,Catarina Madeira,Cláudia Lobato da Silva,Christopher D Porada,Graça Almeida-Porada

Leukemia 28:1716-24 PubMed24476765

2014

A novel TLR-9 agonist C792 inhibits plasmacytoid dendritic cell-induced myeloma cell growth and enhance cytotoxicity of bortezomib.

Applications

Unspecified application

Species

Human

A Ray,Z Tian,D S Das,R L Coffman,P Richardson,D Chauhan,K C Anderson

View all publications

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FITC荧光Anti-TLR9抗体[5G5]

FITC Anti-TLR9 antibody [5G5]

Flow Cytometry (Intracellular) - FITC Anti-TLR9 antibody [5G5] (AB58864)

关键信息

宿主种属

克隆

克隆号

亚型

偶联物

激发波长/发射波长

不含载体蛋白

反应种属

应用

免疫原