FITC荧光Anti-TLR4抗体[HTA125] (ab8378)
![Flow Cytometry - FITC Anti-TLR4 antibody [HTA125] (ab8378)](https://www.abcam.cn/ps/products/8/ab8378/Images/ab8378-14164-anti-tlr4-antibody-hta125-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-TLR4 antibody [HTA125] (ab8378)")
Key features and details
- FITC Mouse monoclonal [HTA125] to TLR4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-TLR4抗体[HTA125]
参阅全部 TLR4 一抗 -
描述
FITC荧光小鼠单克隆抗体[HTA125] to TLR4 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4 has been demonstrated to act as alpha receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.This antibody has been demonstrated to block activation of monocytes with LPS. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Mouse TLR4. Ba/F3 cell line expressing TLR4
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
HTA125 -
骨髓瘤
Sp2/0 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Use 10 µl of this concentration to label 106 cells or 100µl whole blood.
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| 说明 |
|---|
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Flow Cyt
Use at an assay dependent concentration. Use 10 µl of this concentration to label 106 cells or 100µl whole blood.
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
靶标
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功能
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
组织特异性
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
疾病相关
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
结构域
The TIR domain mediates interaction with NOX4. -
翻译后修饰
N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7099 Human
- Omim: 603030 Human
- SwissProt: O00206 Human
- Unigene: 174312 Human
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别名
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
图片
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Flow Cytometry - FITC Anti-TLR4 antibody [HTA125] (ab8378)ab8378 staining TLR4 (FITC) in human peripheral blood monocytes by Flow Cytometry analysis.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab8378 被引用在 9 文献中.
- Yingze Y et al. NOX2-mediated reactive oxygen species are double-edged swords in focal cerebral ischemia in mice. J Neuroinflammation 19:184 (2022). PubMed: 35836200
- Xue Y et al. Resveratrol Confers Vascular Protection by Suppressing TLR4/Syk/NLRP3 Signaling in Oxidized Low-Density Lipoprotein-Activated Platelets. Oxid Med Cell Longev 2021:8819231 (2021). PubMed: 33728029
- Zhang X et al. Phosphatidylserine externalized on the colonic capillaries as a novel pharmacological target for IBD therapy. Signal Transduct Target Ther 6:235 (2021). PubMed: 34131110
- Zhang J et al. Therapeutic effect and mechanism of action of quercetin in a rat model of osteoarthritis. J Int Med Res 48:300060519873461 (2020). PubMed: 31842639
- Al-Rashed F et al. Repetitive Intermittent Hyperglycemia Drives the M1 Polarization and Inflammatory Responses in THP-1 Macrophages Through the Mechanism Involving the TLR4-IRF5 Pathway. Cells 9:N/A (2020). PubMed: 32806763
- Ye Y et al. Meisoindigo Protects Against Focal Cerebral Ischemia-Reperfusion Injury by Inhibiting NLRP3 Inflammasome Activation and Regulating Microglia/Macrophage Polarization via TLR4/NF-?B Signaling Pathway. Front Cell Neurosci 13:553 (2019). PubMed: 31920554
- Hou Y et al. Up-regulation of DcR3 in microbial toxins-stimulated HUVECs involves NF-?B signalling. BMC Biochem 19:13 (2018). PubMed: 30587127
- Velot E et al. Activation of the adenosine-A3 receptor stimulates matrix metalloproteinase-9 secretion by macrophages. Cardiovasc Res : (2008). PubMed: 18653544
- Falcone S et al. Activation of acid sphingomyelinase and its inhibition by the nitric oxide/cyclic guanosine 3',5'-monophosphate pathway: key events in Escherichia coli-elicited apoptosis of dendritic cells. J Immunol 173:4452-63 (2004). PubMed: 15383576