Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

FITC荧光Anti-TLR2抗体[T2.5] (ab59711)

Submit a review Q&A (3)References (7)
Flow Cytometry - FITC Anti-TLR2 antibody [T2.5] (ab59711)
  • Flow Cytometry - FITC Anti-TLR2 antibody [T2.5] (ab59711)

Key features and details

  • FITC Mouse monoclonal [T2.5] to TLR2
  • Suitable for: Flow Cyt
  • Reacts with: Mouse, Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    FITC荧光Anti-TLR2抗体[T2.5]
    参阅全部 TLR2 一抗

  • 描述

    FITC荧光小鼠单克隆抗体[T2.5] to TLR2

  • 宿主

    Mouse

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

  • 经测试应用

    适用于: Flow Cytmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide corresponding to Mouse TLR2.

  • 阳性对照

    • Flow Cyt: RAW264.7 and THP-1 cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab59711于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 10µl for 106 cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
Flow Cyt
Use 10µl for 106 cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

  • 组织特异性

    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

  • 序列相似性

    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • 翻译后修饰

    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD282 antibody
      • CD282 antigen antibody
      • TIL 4 antibody
      • TIL4 antibody
      • TLR 2 antibody
      • TLR2 antibody
      • TLR2_HUMAN antibody
      • Toll like receptor 2 antibody
      • Toll like receptor 2 precursor antibody
      • Toll-like receptor 2 antibody
      • Toll/interleukin 1 receptor like 4 antibody
      • Toll/interleukin 1 receptor like protein 4 antibody
      • Toll/interleukin receptor like protein 4 antibody
      • Toll/interleukin-1 receptor-like protein 4 antibody
      see all

    图片

    • Flow Cytometry - FITC Anti-TLR2 antibody [T2.5] (ab59711)
      Flow Cytometry - FITC Anti-TLR2 antibody [T2.5] (ab59711)

      Flow cytometric analysis of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells staining TLR2 using ab59711 at a 10 µg/mL (blue). Red and black represent the isotype control, cells only.

    • Flow Cytometry - FITC Anti-TLR2 antibody [T2.5] (ab59711)
      Flow Cytometry - FITC Anti-TLR2 antibody [T2.5] (ab59711)
      Overlay histogram showing THP1 cells stained with ab59711 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59711, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 FITC (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    文献 (7)

    发表研究结果有使用 ab59711？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab59711 被引用在 7 文献中.

    • Kornilov FD  et al. The architecture of transmembrane and cytoplasmic juxtamembrane regions of Toll-like receptors. Nat Commun 14:1503 (2023). PubMed: 36932058
    • Moretti IF  et al. Plasmatic membrane toll-like receptor expressions in human astrocytomas. PLoS One 13:e0199211 (2018). PubMed: 29912993
    • Sun W  et al. Crosstalk between TLR2 and Sphk1 in microglia in the cerebral ischemia/reperfusion-induced inflammatory response. Int J Mol Med 40:1750-1758 (2017). PubMed: 29039449
    • Liao K  et al. Cocaine-mediated induction of microglial activation involves the ER stress-TLR2 axis. J Neuroinflammation 13:33 (2016). Flow Cyt ; Mouse . PubMed: 26860188
    • Deng S  et al. Effects of over-expression of TLR2 in transgenic goats on pathogen clearance and role of up-regulation of lysozyme secretion and infiltration of inflammatory cells. BMC Vet Res 8:196 (2012). PubMed: 23082910
    • Selejan S  et al. Ischaemia-induced up-regulation of Toll-like receptor 2 in circulating monocytes in cardiogenic shock. Eur Heart J 33:1085-94 (2012). Flow Cyt ; Human . PubMed: 21998404
    • Ambarus CA  et al. Soluble Immune Complexes Shift the TLR-Induced Cytokine Production of Distinct Polarized Human Macrophage Subsets towards IL-10. PLoS One 7:e35994 (2012). Flow Cyt ; Human . PubMed: 22563430

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Question

    ab59711 gives no signal in IHC-Fr on mouse eye

    Read More
    Answer

    Thank you for your phone call.

    The free of charge replacement antibody you requested, ab24192, will ship on order ***. This antibody has not been validated for use on frozen sections, but may be suitable for that purpose. The antibody may require antigen retrieval for longer PFA fixations. If you are unable to use a shorter fixation method, the attached reference provides details about how to perform heat-mediated antigen retrieval on frozen tissue sections. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Question

    Yes, I am staining wholemount mouse tissue, PFA-fixed.

    Read More
    Answer

    I have found another reference that is more encouraging:

    TLR2 is constitutively expressed within the kidney and participates in ischemic renal injury through both MyD88-dependent and -independent pathways.
    J Immunol. 2007 May 15;178(10):6252-8.

    http://www.jimmunol.org/content/178/10/6252.full

    The mouse tissue was PFA-fixed and embedded frozen in OCT, and it does not appear any antigen retrieval was necessary. It is not clear if the antibody was directly conjugated, or if a secondary antibody was applied.

    Confocal imaging and analysis

    To prepare tissue for confocal imaging, experimental and control groups of WT and TLR2-deficient mice were sacrificed and perfused in situ with 2% paraformaldehyde. Immediately following the perfusion, the kidneys were dissected, removed, and fixed in a solution of 4% paraformaldehyde overnight. The next morning, the kidneys were placed through a sucrose gradient and then frozen in OCT medium (Sakura). Sections (6 μm thick) were stained with anti-TLR2... (see figure 1).

    I found this reference and others by searching via Google Scholar, using the keywords "T2.5" (the clone ID) and TLR2, and formalin or paraformaldehyde.

    I hope this helps. Please let me know if you have any questions.

    Read More

    Question

    Will ab59711 be effective for staining of PFA-fixed (2 hrs) wholemounttissue?

    Read More
    Answer

    Thank you for contacting us. So far, the one reference I have found that used this antibody with frozen tissue sections used acetone as the fixative, not PFA.

    Kidneys were snap frozen in liquid nitrogen and stored at –70°C prior to use. For histological examination of TLR2, kidneys were cut into 6-μm sections, fixed for 10 minutes with ice-cold acetone, and air dried. The sections were incubated in 5% normal goat serum (DakoCytomation) for 10 minutes and then exposed overnight to anti-TLR2 mAb T2.5 in PBS.

    Leemans, J et al; Renal-associated TLR2 mediates ischemia/reperfusion injury in the kidney. J Clin Invest 2005 115: 2894

    I have asked for more data from our source for this clone and will forward this when I receive it. Can you please confirm the species of your samples? I am assuming mouse embryo.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com