FITC荧光Anti-SIRP alpha抗体[BL1H7] (ab23772)
Key features and details
- FITC Mouse monoclonal [BL1H7] to SIRP alpha
- Suitable for: IHC-Fr, IHC-P, Flow Cyt, IP, WB
- Reacts with: Pig
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
概述
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产品名称
FITC荧光Anti-SIRP alpha抗体[BL1H7]
参阅全部 SIRP alpha 一抗 -
描述
FITC荧光小鼠单克隆抗体[BL1H7] to SIRP alpha -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: IHC-Fr, IHC-P, Flow Cyt, IP, WBmore details -
种属反应性
与反应: Pig -
免疫原
Tissue, cells or virus corresponding to Pig SIRP alpha. Porcine alveolar macrophages.
Database link: 494566 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant -
克隆
单克隆 -
克隆编号
BL1H7 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23772于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | ||
| IHC-P | ||
| Flow Cyt | ||
| IP | ||
| WB |
| 说明 |
|---|
应用说明
FACS: Neat-1/10. Use 10µl of the suggested working dilution to 1x106 cells in 100µl.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
组织特异性
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
序列相似性
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
N-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
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别名
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
数据表及文件
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Datasheet download
文献 (3)
ab23772 被引用在 3 文献中.
- Domenech N et al. Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues. Vet Immunol Immunopathol 94:77-81 (2003). PubMed: 12842613
- Alvarez B et al. A porcine cell surface receptor identified by monoclonal antibodies to SWC3 is a member of the signal regulatory protein family and associates with protein-tyrosine phosphatase SHP-1. Tissue Antigens 55:342-51 (2000). PubMed: 10852386
- Bullido R et al. Induction of aggregation in porcine lymphoid cells by antibodies to CD46. Vet Immunol Immunopathol 73:73-81 (2000). PubMed: 10678400
