AB275620

重组FITC荧光Anti-Nicastrin抗体[002]

FITC Anti-Nicastrin antibody [002]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Nicastrin antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NCSTN aa 1-700.

查看别名

KIAA0253, UNQ1874/PRO4317, NCSTN, Nicastrin

1 Images

Flow Cytometry - FITC Anti-Nicastrin antibody [002] (AB275620)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Nicastrin antibody [002] (AB275620)

Flow cytometric analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Nicastrin with ab275620 at 10 μl/Test (Black) compared with isotype control (Grey).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

002

亚型

IgG

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human

应用

Flow Cyt

applications

免疫原

Recombinant Fragment Protein within Human NCSTN aa 1-700. The exact immunogen used to generate this antibody is proprietary information.

Q92542

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>10 μl/Test</p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.09% Sodium azide Constituents: 0.5% BSA

运输条件

Blue Ice

储存信息

Do Not Freeze|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Nicastrin also known as NAS is an integral component of the gamma-secretase complex. This protein interacts mechanically by assisting in substrate recognition and stabilization within the complex. Nicastrin has a molecular weight of approximately 75 kDa. It is predominantly expressed in various tissues including the brain where it plays significant role in neuronal functions.

Biological function summary

Nicastrin functions as a part of the gamma-secretase complex which also includes presenilin anterior pharynx defective 1 (APH-1) and presenilin enhancer 2 (PEN-2). This complex is essential for intramembrane proteolysis of type I membrane proteins such as amyloid precursor protein (APP) and Notch. The activity of nicastrin influences processes like synaptic organization and neuronal development.

Pathways

Gamma-secretase activity facilitated by nicastrin is central in the Notch signaling pathway and the amyloidogenic pathway. In the Notch pathway it helps release the Notch intracellular domain important for T-cell development. In the amyloidogenic pathway the complex processes APP contributing to amyloid-beta peptide formation. Beta-secretase (BACE1) also plays an important role in this pathway acting upstream of gamma-secretase.

The imbalance in nicastrin function connects it to Alzheimer's disease and certain cancers. Its role in amyloid-beta peptide production links nicastrin to Alzheimer's disease pathogenesis. In cancer the deregulation of Notch signaling in which nicastrin is involved influences tumor progression and survival. The interaction of nicastrin with presenilin is particularly notable in these conditions further emphasizing its significance in disease mechanisms.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed : 10993067, PubMed : 12679784, PubMed : 25043039, PubMed : 26280335, PubMed : 30598546, PubMed : 30630874). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.

See full target information NCSTN

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular genetics & genomic medicine 12:e70030 PubMed39600113

2024

Pathogenicity of the LDLR c.97C>T (p.Gln33Ter) Mutation in Familial Hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Kaihan Wang,Tingting Hu,Mengmeng Tai,Yan Shen,Shaoyi Lin,Yongjuan Guo,Xiaomin Chen

View all publications

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重组FITC荧光Anti-Nicastrin抗体[002]

FITC Anti-Nicastrin antibody [002]

Flow Cytometry - FITC Anti-Nicastrin antibody [002] (AB275620)

关键信息

宿主种属

克隆

克隆号

亚型

偶联物

激发波长/发射波长

不含载体蛋白

反应种属

应用

免疫原