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Tags & Cell Markers Epitope Tags Myc Tag

FITC荧光Anti-Myc tag抗体(ab1263)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (6)

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Immunocytochemistry/ Immunofluorescence - FITC Anti-Myc tag antibody (ab1263)

    Key features and details

    • FITC Goat polyclonal to Myc tag
    • Suitable for: ICC, ICC/IF
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG

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    概述

    • 产品名称

      FITC荧光Anti-Myc tag抗体
      参阅全部 Myc tag 一抗
    • 描述

      FITC荧光山羊多克隆抗体to Myc tag
    • 宿主

      Goat
    • 偶联物

      FITC. Ex: 493nm, Em: 528nm
    • 经测试应用

      适用于: ICC, ICC/IFmore details
    • 免疫原

      Full length native protein

      EQKLISEEDL

      (c-myc) (purified): conjugated to KLH.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      F/P ratio is 3 to 7.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C.
    • 存储溶液

      Preservative: 0.1% Sodium azide
      Constituent: 0.2% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Tags & Cell Markers
      • Epitope Tags
      • Myc Tag

    相关产品

    • Isotype control

      • FITC Goat IgG - Isotype control (ab37374)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1263于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC
    1/200 - 1/2000.
    ICC/IF (1)
    1/200. (see Abreview).
    说明
    ICC
    1/200 - 1/2000.
    ICC/IF
    1/200. (see Abreview).

    靶标

    • 相关性

      Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
    • 细胞定位

      Nuclear
    • 别名

      • c-myc tag antibody
      • Myc Epitope Tag antibody

    图片

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-Myc tag antibody (ab1263)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-Myc tag antibody (ab1263)Image from Poncet D et al, J Cell Biol. 2006 Sep 25;174(7):985-96. Epub 2006 Sep 18, Fig 3D. DOI 10.1083/jcb.200604069
      ab1263 staining Myc tag (red) in HeLa cells by Immunocytochemistry/ Immunofluorescence.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (6)

    发表研究结果有使用 ab1263?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1263 被引用在 6 文献中.

    • Baud C  et al. Translocation path of a substrate protein through its Omp85 transporter. Nat Commun 5:5271 (2014). PubMed: 25327833
    • Zou S  et al. A novel actin-binding domain on Slo1 calcium-activated potassium channels is necessary for their expression in the plasma membrane. Mol Pharmacol 73:359-68 (2008). PubMed: 17989352
    • Zou S  et al. The beta 1 subunit of L-type voltage-gated Ca2+ channels independently binds to and inhibits the gating of large-conductance Ca2+-activated K+ channels. Mol Pharmacol 73:369-78 (2008). PubMed: 17989350
    • Kim EY  et al. Nephrin binds to the COOH terminus of a large-conductance Ca2+-activated K+ channel isoform and regulates its expression on the cell surface. Am J Physiol Renal Physiol 295:F235-46 (2008). ICC/IF . PubMed: 18480178
    • Kim EY  et al. Interactions with filamin A stimulate surface expression of large-conductance Ca2+-activated K+ channels in the absence of direct actin binding. Mol Pharmacol 72:622-30 (2007). PubMed: 17586600
    • Poncet D  et al. Cytopathic effects of the cytomegalovirus-encoded apoptosis inhibitory protein vMIA. J Cell Biol 174:985-96 (2006). PubMed: 16982800

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myc tag antibody (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Mesenchymal stem cell)
    Specification
    Mesenchymal stem cell
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Mal Niladri

    Verified customer

    提交于 Nov 08 2006

    Question

    We are using ab1263 [anti-Myc, FITC-conjugated] extensively [and many other Abcam products, including custom Ab prep.] For our use in FRET, we need to know the affinity value of ab1263 for its peptide.Can you give me that value ,please? [That is not covered in the datasheet or technical enquiry page for 1263].

    Read More

    Abcam community

    Verified customer

    Asked on Nov 05 2004

    Answer

    Unfortunately we do not have that information.

    Read More

    Abcam Scientific Support

    回复于 Nov 05 2004

    Question

    hi - I have a question about the myc tag antibody, catalogue number ab1263. I it FITC conjugated - but you say it can be used in Immunohistochemistry - can it be used using the traditional ABC method? or just immunofluorescence? Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 27 2003

    Answer

    We suggest that the FITC conjugated antibody should be used for immunofluorescence.

    Read More

    Abcam Scientific Support

    回复于 Dec 02 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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