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AB314952

FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control

FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control

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FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control Suitable for Flow Cyt (Intra) and reacts with samples.
1 Images
Flow Cytometry (Intracellular) - FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB314952)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB314952)

Flow cytometry overlay histogram showing HeLa cells stained with ab119688 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 90% methanol for 30 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab119688) (1x 106 in 100μl at 1.0μg/ml) for 30min at 22°C.

Isotype control antibody (black line) was ab314952 (1.0μg/ml (1/500)) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

不同偶联物与剂型 (3)

  • 578 PE

    PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control

  • Unconjugated

    Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control

  • Carrier free

    Mouse IgG1 monoclonal [R312-MouseIgG1] - Isotype control - BSA and Azide free

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

R312-MouseIgG1

亚型

IgG1

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

No

应用

Flow Cyt (Intra)

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Mouse IgG1 also known as immunoglobulin G1 is an important antibody isotype in mice. It has a molecular mass of approximately 150 kDa. Mouse IgG1 is found in the serum and extracellular fluids where it helps to mediate immune functions. This antibody is produced by plasma cells which originate from B lymphocytes. Its structure is comprised of two heavy chains and two light chains forming a Y-shaped molecule that facilitates antigen binding.
Biological function summary

Immunoglobulin G1 plays a significant role in adaptive immunity as it can engage with different cellular receptors and complement proteins to mediate immune responses. It does not function as part of a complex by itself but rather interacts with other immune system components to execute its tasks. Mouse IgG1 is known for its ability to neutralize pathogens agglutinate invading microbes and opsonize them for enhanced phagocytosis by immune cells like macrophages and neutrophils.

Pathways

The signaling processes associated with mouse IgG1 involve pathways such as the Fc gamma receptor pathway and the classical complement pathway. Engagement of its Fc region with Fc gamma receptors on the surface of immune cells leads to effector functions like phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). In these pathways proteins like complement component C1q and other Fc gamma receptor subtypes play key roles in modulating the immune responses initiated by IgG1.

Immunoglobulin G1 is relevant in conditions like autoimmune diseases and allergic reactions where inappropriate immune activation or regulation can be observed. In autoimmune diseases for example IgG1 can form immune complexes that deposit in tissues and activate inflammatory cascades involving proteins like complement factors and other immunoglobulin subclasses. Its involvement in allergic responses often involves IgG1 competing with IgE antibodies for antigen binding altering the allergic response dynamics.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com