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FITC荧光Anti-M6PR (cation independent)抗体[MEM-238] (ab58841)

Reviews (1)Q&A (1)References (2)
Flow Cytometry - FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)
  • Flow Cytometry (Intracellular) - FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)

Key features and details

  • FITC Mouse monoclonal [MEM-238] to M6PR (cation independent)
  • Suitable for: Flow Cyt, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Biotin PE Unconjugated

概述

  • 产品名称

    FITC荧光Anti-M6PR (cation independent)抗体[MEM-238]
    参阅全部 M6PR (cation independent) 一抗

  • 描述

    FITC荧光小鼠单克隆抗体[MEM-238] to M6PR (cation independent)

  • 宿主

    Mouse

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

  • 经测试应用

    适用于: Flow Cyt, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human
    预测可用于: Non human primates

  • 免疫原

    Tissue, cells or virus corresponding to Human M6PR (cation independent).
    Database link: P11717

  • 表位

    ab58841 recognizes an epitope between domains 2 and 5 of Mannose 6 Phosphate Receptor (Cation independent) (CD222).

  • 阳性对照

    • Flow Cyt: Human Peripheral whole blood, HeLa cells. Flow Cyt (Intra): HeLa Cells.

  • 常规说明

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab58841于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 8.3µl for 106 cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
Flow Cyt
Use 8.3µl for 106 cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.

  • 序列相似性

    Belongs to the MRL1/IGF2R family.
    Contains 1 fibronectin type-II domain.

  • 结构域

    Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.

  • 细胞定位

    Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 300 kDa mannose 6 phosphate receptor antibody
      • 300 kDa mannose 6-phosphate receptor antibody
      • Cation independent mannose 6 phosphate receptor antibody
      • Cation-independent mannose-6-phosphate receptor antibody
      • CD222 antibody
      • CD222 antigen antibody
      • CI Man 6 P receptor antibody
      • CI Man-6-P receptor antibody
      • CI MPR antibody
      • CI-M6PR antibody
      • CI-MPR antibody
      • CIMPR antibody
      • IGF 2 receptor antibody
      • IGF 2R antibody
      • IGF II receptor antibody
      • IGF-II receptor antibody
      • IGF2 receptor antibody
      • Igf2r antibody
      • Insulin like growth factor 2 receptor antibody
      • Insulin like growth factor II receptor antibody
      • Insulin-like growth factor 2 receptor antibody
      • Insulin-like growth factor II receptor antibody
      • M6P R antibody
      • M6P/IGF2 receptor antibody
      • M6P/IGF2R antibody
      • M6PR antibody
      • mannose 6 phosphate receptor antibody
      • mannose 6 phosphate receptor, cation independent antibody
      • MPR 300 antibody
      • MPR300 antibody
      • MPRI antibody
      • MPRI_HUMAN antibody
      see all

    图片

    • Flow Cytometry - FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)
      Flow Cytometry - FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)

      Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD222 (MEM-238) FITC antibody (ab58841) (red, concentration in sample 3 μg/ml) and mouse IgG1 isotype control (MOPC-21) FITC antibody (blue, concentration in sample 3 μg/ml).

    • Flow Cytometry (Intracellular) - FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)
      Flow Cytometry (Intracellular) - FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)
      Overlay histogram showing HeLa cells stained with ab58841 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58841, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

    文献 (2)

    发表研究结果有使用 ab58841？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab58841 被引用在 2 文献中.

    • Laube F Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression. Anticancer Res 29:1383-8 (2009). Inhibition Assay ; Human . PubMed: 19414391
    • Leksa V  et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). WB, Flow Cyt ; Human . PubMed: 12189157

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mannose 6 Phosphate Receptor (Cation independent) antibody [MEM-238] (FITC)

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample
    Human Cell (HEK293, HeLa)
    Specification
    HEK293, HeLa
    Permeabilization
    Yes - 0.5% NP-40, 1%BSA in PBS
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Monica Pons

    Verified customer

    提交于 Oct 01 2013

    Question

    Where is the epitope that the antibody recognizes located? Is it extracellular?

    Read More
    Answer

    Thank you for contacting us. The following two papers include protocols with this clone, MEM-238, which indicate that the antibody recognizes an extracellular epitope. This will also be the case for the FITC conjugate, ab58441. (The unconjugated version is catalogue ID ab8093). Leksa V et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). WB, Flow Cyt; Human. PubMed: 12189157 Laube F Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression. Anticancer Res 29:1383-8 (2009). Inhib; Human. PubMed: 19414391   The flow cytometry protocol in the Leska paper used live cells, without permeabilization, and the inhibition assay in the Laube paper also used live cells. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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