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FITC荧光Anti-Ly6g抗体[RB6-8C5] (ab25024)

Reviews (6)Q&A (4)References (19)
Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
  • Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
  • Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
  • Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)

Key features and details

  • FITC Rat monoclonal [RB6-8C5] to Ly6g
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

APC Biotin PE PE/Cy7® redFluor™ 710 Unconjugated violetFluor™ 450

概述

  • 产品名称

    FITC荧光Anti-Ly6g抗体[RB6-8C5]
    参阅全部 Ly6g 一抗

  • 描述

    FITC荧光大鼠单克隆抗体[RB6-8C5] to Ly6g

  • 宿主

    Rat

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

  • 特异性

    ab25024 recognises mouse Mouse Ly-6G.

  • 经测试应用

    适用于: Flow Cytmore details

  • 种属反应性

    与反应: Mouse

  • 免疫原

    Tissue, cells or virus corresponding to Ly6g. Mouse granulocytes

  • 阳性对照

    • Rat IgG2b (FITC) isotype control

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab25024于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (5)
1/100.

ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
说明
Flow Cyt
1/100.

ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性

    Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.

  • 细胞定位

    Attached to the membrane by a GPI-anchor.

  • 数据库链接

    • 别名

      • Gr-1 antibody
      • Gr1 antibody
      • Ly-6G.1 antibody
      • Ly6G antibody
      • Lymphocyte antigen 6 complex locus G antibody
      • Lymphocyte antigen 6G antibody
      see all

    图片

    • Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
      Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)

      Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 0.5µg (right panel), Rat IgG2b (FITC) isotype control at 0.5µg (left panel).

    • Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
      Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)

      BALB/c bone marrow cells were stained with either rat IgG2b-FITC (as an isotype control) or rat anti-mouse Ly-6G-FITC (ab25024) at a concentration of 1ug/106 cells.  Large cells were then gated and analyzed on a flow cytometer.

    • Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
      Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)

      Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 at 0.5µg.

    • Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
      Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)Image courtesy of an anonymous Abreview.

      ab25024 at a 1/100 dilution detecting Ly6g (Gr1) in murine blood cells by Flow Cytometry. Gating strategy was for monocytes.

      See Abreview

    文献 (19)

    发表研究结果有使用 ab25024？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab25024 被引用在 19 文献中.

    • Fan Z  et al. Sympathetic Nerve-Mediated Fellow Eye Pain During Sequential Cataract Surgery by Regulating Granulocyte Colony Stimulating Factor CSF3. Front Cell Neurosci 16:841733 (2022). PubMed: 35281296
    • Tian S  et al. Curcumin inhibits the growth of liver cancer by impairing myeloid-derived suppressor cells in murine tumor tissues. Oncol Lett 21:286 (2021). PubMed: 33732362
    • Nugraha B  et al. The p38/MK2 Axis in Monocytes of Fibromyalgia Syndrome Patients: An Explorative Study. Medicina (Kaunas) 57:N/A (2021). PubMed: 33921654
    • Lv D  et al. A novel cell-based assay for dynamically detecting neutrophil extracellular traps-induced lung epithelial injuries. Exp Cell Res 394:112101 (2020). PubMed: 32474064
    • Ding F  et al. microRNA-375 released from extracellular vesicles of bone marrow mesenchymal stem cells exerts anti-oncogenic effects against cervical cancer. Stem Cell Res Ther 11:455 (2020). PubMed: 33109266
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 10 Abreviews or Q&A

    Flow Cytometry abreview for Anti-Ly6g antibody [RB6-8C5] (FITC)

     Poor
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Dog Cell (from whole blood)
    Specification
    from whole blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive cells
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 06 2012

    Flow Cytometry abreview for Anti-Ly6g antibody [RB6-8C5] (FITC)

     Excellent
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rat Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Permeabilization
    No
    Gating Strategy
    alive macrophages
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 19 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Ly6g antibody [RB6-8C5] (FITC)

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Spleen from immune mouse)
    Specification
    Spleen from immune mouse
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 16 2012

    Flow Cytometry abreview for Anti-Ly6g antibody [RB6-8C5] (FITC)

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Cynomolgus Monkey Cell (Whole blood)
    Specification
    Whole blood
    Preparation
    Cell harvesting/tissue preparation method: After red blood cell lysis
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive monocytes
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 16 2012

    Flow Cytometry abreview for Anti-Ly6g antibody [RB6-8C5] (FITC)

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (LPS activated monocytes)
    Specification
    LPS activated monocytes
    Permeabilization
    No
    Gating Strategy
    alive cells
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 23 2011

    Flow Cytometry abreview for Anti-Ly6g antibody [RB6-8C5] (FITC)

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (cells from blood)
    Specification
    cells from blood
    Permeabilization
    No
    Gating Strategy
    monocytes
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 23 2011

    Question

    Our facs staining protocol looks like this: 1) incubate cells (100ul whole blood, or 1 x 10^6 splenocytes) with anti-CD32 for 10 minutes to block 2) add primary staining antibodies for 30 minutes, 4 degrees, dark: we use 0.75ul stock solution per sample of FITC anti-Ly6G from Abcam 3) wash twice with 1 ml facs buffer 4) secondary stain as needed, 30 minutes, 4 degrees, dark 5) (For PBMCs) lyse 3 minutes room temp in ACK, spin 6) Wash in 1 ml facs buffer 7) fix in 1%PFA in PBS 8) run samples on FACS Aria

    Read More
    Answer

    Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab25024. I would also appreciate if you can confirm some further details: 1) I see in your details that you are using 0.75ul (0.375ug) of antibody for your stain. We recommend use of 1ug (2ul) of antibody per 1x10^6 cells, have you attempted to use a higher concentration (2ul) per sample? 2) Longer incubation may be necessary for optimal binding of the antibody in your samples. We would recommend trying a 45 minute of 1 hour incubation to give this item more time to bind the expressed Ly6g. 3) Wash the cells 3 X by centrifugation in FACS buffer at 400 g for 5 minutes and resuspend them in 500 µl to 1 ml of ice cold FACS buffer and store cold in dark until analysis. 4) The light fixation may also be interfering with this product, have you have the chance to run this without using 1% pfa?

    Read More

    Question

    ReviewID: xxx AbID: 25024, Anti-Ly6g antibody [RB6-8C5] (FITC) Rating: Poor Image: Anti-Ly6g antibody [RB6-8C5] (FITC) for Flow Cytometry (Dog) Sample: Species: Dog Type: Cell Specification: from whole blood Preparation: Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1% Application: Application: Fixation: Paraformaldehyde Permeabilization: No Gating Strategy: alive cells Other detail: Dilution: 1/100 Incubation time: 1 hour at 4°C Diluent: PBS/BSA 1% Secondary Antibody: Name: None used Conjugation: Additional Data:

    Read More
    Answer

    Thank you for submitting an Abreview for ab25024. As you may have noticed, your review has now been published on our website.
    Since you obtained poor results using the antibody in an untested species (dog), we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.
    Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:
    - fix with PFA after incubation with the primary antibody - if you haven't done that already.
    - incubate longer with the primary antibody (e.g. 2-3 h at RT or overnight at 4 degree)
    It could also just be the case that the antibody does not work for dog samples due to potential low homology.
    If you decide to follow these suggestions, please let me know if they are helpful.
    I wish you good luck with your research.

    Read More

    Question

    What isotype control was used in the data shown on the online datasheet?

    Read More
    Answer

    Thank you for your patience.


    I have now had the Rat IgG1 (FITC) isotype control added to our catalog, ab128477.


    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Question

    What isotype control was used in the data shown on the online datasheet?

    Read More
    Answer

    Thank you for contacting Abcam regarding ab25024.


    I am working to have the appropriate isotype control added to our catalog for you to purchase. I will provide the ordering details as soon as they are available.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Read More

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