AB25024

FITC荧光Anti-Ly6g抗体[RB6-8C5]

Name: FITC Anti-Ly6g抗体[RB6-8C5] (ab25024)| Abcam中文官网
Brand: Abcam Limited
SKU: AB25024
Price: 5126 CNY
Availability: InStock
Rating: 4 (6 reviews)

FITC Anti-Ly6g antibody [RB6-8C5]

(6 Reviews)

(25 Publications)

Rat Monoclonal Ly6g antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 25 publications.

查看别名

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g

4 Images

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

Flow Cyt

AbReview27588****

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

ab25024 at a 1/100 dilution detecting Ly6g (Gr1) in murine blood cells by Flow Cytometry. Gating strategy was for monocytes.

Image courtesy of an anonymous Abreview.

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 0.5μg (right panel), Rat IgG2b (FITC) isotype control at 0.5μg (left panel).

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

BALB/c bone marrow cells were stained with either rat IgG2b-FITC (as an isotype control) or rat anti-mouse Ly-6G-FITC (ab25024) at a concentration of 1ug/10⁶ cells. Large cells were then gated and analyzed on a flow cytometer.

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 at 0.5μg.

不同偶联物与剂型 (6)

Unconjugated

Anti-Ly6g + Ly6c antibody [RB6-8C5]
redFluor™ 710

redFluor™ 710 Anti-Ly6g antibody [RB6-8C5]
578 PE

PE Anti-Ly6g antibody [RB6-8C5]
660 APC

APC Anti-Ly6g antibody [RB6-8C5]
778 PE/Cy7®

PE/Cy7® Anti-Ly6g antibody [RB6-8C5]
450 violetFluor™ 450

violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5]

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

RB6-8C5

亚型

IgG2b

轻链类型

kappa

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Mouse

应用

Flow Cyt

applications

特异性

ab25024 recognises mouse Mouse Ly-6G.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/fitc-rat-igg2b-kappa-monoclonal-eb149-10h5-isotype-control-ab136125'>ab136125</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.

Biological function summary

Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.

Pathways

Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.

Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Ly6g

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文献 (25)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed40709382

2025

Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Chao Yang,Ya Yue,Yi-Ting Wang,John Y Chiang,Ben-Chung Cheng,Tsuen-Wei Hsu,Yi-Ling Chen,Yi-Chen Li,Hon-Kan Yip

iScience 28:112291 PubMed40241756

2025

Somatic modulates mitochondrial function in airway multiciliated cells and exacerbates influenza pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jhonatan Henao Vasquez,Jin Yuan,Chi Jing Leow,Erin Crossey,Fengzhi Shao,Senegal Carty,Viviana A Dominguez,Ming Lo,Joseph P Mizgerd,Jessica L Fetterman,Nelson C Lau,Alan Fine,Matthew R Jones

Acta neuropathologica communications 13:33 PubMed39966968

2025

Blocking peptidyl arginine deiminase 4 confers neuroprotective effect in the post-ischemic brain through both NETosis-dependent and -independent mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Song-I Seol,Sang-A Oh,Dashdulam Davaanyam,Ja-Kyeong Lee

Molecular medicine (Cambridge, Mass.) 31:46 PubMed39910417

2025

Platelet-derived HMGB1 induces NETosis, exacerbating brain damage in the photothrombotic stroke model.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-A Oh,Song-I Seol,Dashdulam Davaanyam,Seung-Woo Kim,Ja-Kyeong Lee

Scientific reports 14:17241 PubMed39060348

2024

Progesterone modulates the immune microenvironment to suppress ovalbumin-induced airway inflammation by inhibiting NETosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Wang,Feng-Ying Huang,Shu-Zhen Dai,Yongshu Fu,Xiangdong Zhou,Cai-Chun Wang,Guang-Hong Tan,Qi Li

International journal of molecular sciences 23: PubMed36362052

2022

Inhibition of Netosis with PAD Inhibitor Attenuates Endotoxin Shock Induced Systemic Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Huanling Yao,Guojie Cao,Zheng Liu,Yue Zhao,Zhanchao Yan,Senzhen Wang,Yuehua Wang,Zhengwei Guo,Yanming Wang

Frontiers in cellular neuroscience 16:841733 PubMed35281296

2022

Sympathetic Nerve-Mediated Fellow Eye Pain During Sequential Cataract Surgery by Regulating Granulocyte Colony Stimulating Factor CSF3.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Fan,Caixia Fan,Benxiang Qi,Bin Zhang,Wenfeng Li,Xia Qi,Xiaomin Liu,Bi Ning Zhang,Yusen Huang

Medicina (Kaunas, Lithuania) 57: PubMed33921654

2021

The p38/MK2 Axis in Monocytes of Fibromyalgia Syndrome Patients: An Explorative Study.

Applications

Unspecified application

Species

Unspecified reactive species

Boya Nugraha,Renate Scheibe,Christoph Korallus,Matthias Gaestel,Christoph Gutenbrunner

Oncology letters 21:286 PubMed33732362

2021

Curcumin inhibits the growth of liver cancer by impairing myeloid-derived suppressor cells in murine tumor tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Tian,Liu Liao,Qing Zhou,Xiaodi Huang,Piao Zheng,Yinmei Guo,Tianhao Deng,Xuefei Tian

Frontiers in medicine 7:577578 PubMed33425934

2020

Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-25 Regulates the Ubiquitination and Degradation of Runx2 by SMURF1 to Promote Fracture Healing in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yikun Jiang,Jun Zhang,Zhengwei Li,Guoliang Jia

View all publications

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FITC荧光Anti-Ly6g抗体[RB6-8C5]