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FITC荧光Anti-Integrin beta 4抗体[450-9D] (ab22486)

Submit a review Q&A (2)References (3)
Immunohistochemistry (Frozen sections) - FITC Anti-Integrin beta 4 antibody [450-9D] (ab22486)

    Key features and details

    • FITC Mouse monoclonal [450-9D] to Integrin beta 4
    • Suitable for: IHC-Fr
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    概述

    • 产品名称

      FITC荧光Anti-Integrin beta 4抗体[450-9D]
      参阅全部 Integrin beta 4 一抗

    • 描述

      FITC荧光小鼠单克隆抗体[450-9D] to Integrin beta 4

    • 宿主

      Mouse

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    • 特异性

      ab22486 recognises the integrin beta 4 expressed on epithelial cells, Schwann cells and various tumour cell lines.

    • 经测试应用

      适用于: IHC-Frmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Full length native protein (purified) (Human): Purified a6b4 integrin from A431 cells

    • 表位

      the epitope is in the extracellular region of Integrin beta 4

    • 阳性对照

      • Human tonsil

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...

    • 纯度

      Protein G purified

    • 纯化说明

      ab22486 has been purified by affinity chromatography on Protein G from tissue culture supernatant.

    • 克隆

      单克隆

    • 克隆编号

      450-9D

    • 骨髓瘤

      Sp2/0

    • 同种型

      IgG1

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab22486于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-Fr
    Use at an assay dependent concentration.
    说明
    IHC-Fr
    Use at an assay dependent concentration.

    靶标

    • 功能

      Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.

    • 组织特异性

      Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.

    • 疾病相关

      Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
      Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.

    • 序列相似性

      Belongs to the integrin beta chain family.
      Contains 1 Calx-beta domain.
      Contains 4 fibronectin type-III domains.
      Contains 1 PSI domain.
      Contains 1 VWFA domain.

    • 结构域

      The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD 104 antibody
        • CD104 antibody
        • CD104 antigen antibody
        • gp150 antibody
        • Integrin beta 4 subunit antibody
        • Integrin beta-4 antibody
        • ITB4_HUMAN antibody
        • ITG B4 antibody
        • ITGB 4 antibody
        • Itgb4 antibody
        see all

      图片

      • Immunohistochemistry (Frozen sections) - FITC Anti-Integrin beta 4 antibody [450-9D] (ab22486)
        Immunohistochemistry (Frozen sections) - FITC Anti-Integrin beta 4 antibody [450-9D] (ab22486)

        Immunohistochemistry (Frozen sections) analysis of human tonsil tissue section labeling Integrin beta 4 with ab22486. 

      文献 (3)

      发表研究结果有使用 ab22486？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab22486 被引用在 3 文献中.

      • Gamage TKJB  et al. Human trophoblasts are primarily distinguished from somatic cells by differences in the pattern rather than the degree of global CpG methylation. Biol Open 7:N/A (2018). PubMed: 30026266
      • James JL  et al. Isolation and characterisation of a novel trophoblast side-population from first trimester placentae. Reproduction 150:449-62 (2015). PubMed: 26248480
      • DaSilva-Arnold S  et al. Differentiation of first trimester cytotrophoblast to extravillous trophoblast involves an epithelial-mesenchymal transition. Placenta 36:1412-8 (2015). PubMed: 26545962

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Question

      BATCH NUMBER -- 191982. -- ORDER NUMBER -- order was placed on 23rd or 24th of June-- DESCRIPTION OF THE PROBLEM No bands. When I used a rabbit polyclonal antibody against the same I could get proper signal SAMPLE Human whole cell lysate from C4-2 PRIMARY ANTIBODY ABCAM mouse monoclonal DETECTION METHOD Pierce POSITIVE AND NEGATIVE CONTROLS USED Nothing ANTIBODY STORAGE CONDITIONS As recommended SAMPLE PREPARATION RIPA lysis buffer from SCBT AMOUNT OF PROTEIN LOADED 60 micrograms ELECTROPHORESIS/GEL CONDITIONS Reducing, 8% 50 volts for 45 minutes and 2and half hour at 80 volts TRANSFER AND BLOCKING CONDITIONS 0.05% SDS in TRIS-Glysine buffer at 4 degree centigrade and 380mA for 21/2 hours( This is very standard procedure I followd for all my investigation with integrins. SECONDARY ANTIBODY Mouse IgG SCBT HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? one HAVE YOU RUN A "NO PRIMARY" CONTROL? No WHAT STEPS HAVE YOU ALTERED? Nothing. I followed the same protocol that has been working for me with polyconal antibodies.

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      Answer

      Further to previous correspondence I have been in touch with the source of this antibody. I am concerned that this antibody has in fact NOT been tested and shown to work by western blotting. It is FITC labeled and therefore my concern was that the fluorophore label was occluding the capacity for the secondary to recognise the primary antibody. Given that it is yet to be tested I am certainly prepared to offer you a credit note against this purchase provided that the antibody was purchased within the past 90 days. If this is the case please email me details of the order including the order number and date of purchase. I look forward to hearing from you.

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      Question

      BATCH NUMBER -- 191982. -- ORDER NUMBER -- order was placed on 23rd or 24th of June-- DESCRIPTION OF THE PROBLEM No bands. When I used a rabbit polyclonal antibody against the same I could get proper signal SAMPLE Human whole cell lysate from C4-2 PRIMARY ANTIBODY ABCAM mouse monoclonal DETECTION METHOD Pierce POSITIVE AND NEGATIVE CONTROLS USED Nothing ANTIBODY STORAGE CONDITIONS As recommended SAMPLE PREPARATION RIPA lysis buffer from SCBT AMOUNT OF PROTEIN LOADED 60 micrograms ELECTROPHORESIS/GEL CONDITIONS Reducing, 8% 50 volts for 45 minutes and 2and half hour at 80 volts TRANSFER AND BLOCKING CONDITIONS 0.05% SDS in TRIS-Glysine buffer at 4 degree centigrade and 380mA for 21/2 hours( This is very standard procedure I followd for all my investigation with integrins. SECONDARY ANTIBODY Mouse IgG SCBT HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? one HAVE YOU RUN A "NO PRIMARY" CONTROL? No WHAT STEPS HAVE YOU ALTERED? Nothing. I followed the same protocol that has been working for me with polyconal antibodies.

      Read More
      Answer

      Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I appreciate the controls that you have in place and that you are detecting Integrin beta 4 using an alternatively sourced antibody. Please can you detail the antibody dilutions that you have applied the antibody at and whether the alternative antibody was applied in parallel to control for variation in your experimental procedure. I am in touch with the lab to determine the conditions employed as my concern is that given this antibody is FITC conjugated and may occlude the binding of a secondary antibody. I look forward to hearing from you.

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