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FITC荧光Anti-Integrin alpha 6抗体[450-30A] (ab30496)

Submit a review Q&A (1)References (4)
Flow Cytometry - FITC Anti-Integrin alpha 6 antibody [450-30A] (ab30496)

    Key features and details

    • FITC Mouse monoclonal [450-30A] to Integrin alpha 6
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    概述

    • 产品名称

      FITC荧光Anti-Integrin alpha 6抗体[450-30A]
      参阅全部 Integrin alpha 6 一抗

    • 描述

      FITC荧光小鼠单克隆抗体[450-30A] to Integrin alpha 6

    • 宿主

      Mouse

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Full length native protein (Human): alpha 6 beta 4 integrin purified from A431 cells

    • 阳性对照

      • human peripheral blood lymphocytes

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab30496于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use 10µl for 106 cells.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 
    说明
    Flow Cyt
    Use 10µl for 106 cells.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

    靶标

    • 功能

      Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).

    • 组织特异性

      Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).

    • 疾病相关

      Epidermolysis bullosa letalis, with pyloric atresia

    • 序列相似性

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.

    • 翻译后修饰

      Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
      In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
      Palmitoylation by DHHC3 enhances stability and cell surface expression.

    • 细胞定位

      Cell membrane. Cell membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Alpha6p antibody
        • CD49 antigen-like family member F antibody
        • CD49f antibody
        • Integrin alpha6B antibody
        • Integrin, alpha 6 antibody
        • ITA6_HUMAN antibody
        • ITGA6 antibody
        • ITGA6B antibody
        • Processed integrin alpha-6 antibody
        • VLA 6 antibody
        • VLA-6 antibody
        see all

      图片

      • Flow Cytometry - FITC Anti-Integrin alpha 6 antibody [450-30A] (ab30496)
        Flow Cytometry - FITC Anti-Integrin alpha 6 antibody [450-30A] (ab30496)
        Flow cytometric analysis of peripheral blood lymphocytes with 10µl for 106 cells of anti human Integrin alpha 6 FITC conjugated (ab30496).

      文献 (4)

      发表研究结果有使用 ab30496？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab30496 被引用在 4 文献中.

      • Woappi Y  et al. Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity. J Vis Exp N/A:N/A (2021). PubMed: 33586700
      • Woappi Y  et al. Self-assembling 3D spheroid cultures of human neonatal keratinocytes have enhanced regenerative properties. Stem Cell Res 49:102048 (2020). PubMed: 33128954
      • Sobral LM  et al. SET/I2PP2A overexpression induces phenotypic, molecular, and metabolic alterations in an oral keratinocyte cell line. FEBS J 284:2774-2785 (2017). PubMed: 28636114
      • Calenic B  et al. Magnetic separation and characterization of keratinocyte stem cells from human gingiva. J Periodontal Res 45:703-8 (2010). ICC/IF, Flow Cyt ; Human . PubMed: 20546107

      客户评价及客户问答

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      Question

      I would like to test ab30496 for use on mouse samples.

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      Answer

      DISCOUNT CODE: ***
      Expiration date: June 15th, 2012

      I am very pleased to hear you would like to accept our offer and test ab30496 in mouse. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com