FITC荧光Anti-IL-6R抗体[B-R6] (ab27321)
![Flow Cytometry - FITC Anti-IL-6R antibody [B-R6] (ab27321)](https://www.abcam.cn/ps/products/27/ab27321/Images/ab27321-38081-anti-il-6r-antibody-b-r6-fitc-flow-cytometry.gif "Flow Cytometry - FITC Anti-IL-6R antibody [B-R6] (ab27321)")
Key features and details
- FITC Mouse monoclonal [B-R6] to IL-6R
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-IL-6R抗体[B-R6]
参阅全部 IL-6R 一抗 -
描述
FITC荧光小鼠单克隆抗体[B-R6] to IL-6R -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
Recognises the Interleukin-6 Ra subunit, IL-6 Ra, IL-6 R, a 80 kDa protein.
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经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human IL-6R. gp80 transfected CHO cell line
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阳性对照
- Cells from U266 cell line.
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常规说明
This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.30
Preservative: 0.09% Sodium azide
Constituents: 94.91% PBS, 5% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
B-R6 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27321于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity. -
组织特异性
Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum. -
序列相似性
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
结构域
The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. -
翻译后修饰
A short soluble form may also be released from the membrane by proteolysis. -
细胞定位
Secreted and Basolateral cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3570 Human
- Omim: 147880 Human
- SwissProt: P08887 Human
- Unigene: 709210 Human
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别名
- CD 126 antibody
- CD126 antibody
- CD126 antigen antibody
see all
图片
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Flow Cytometry - FITC Anti-IL-6R antibody [B-R6] (ab27321)
ab27321 at 10µl/106 cells staining IL6R in U266 cell line by Flow Cytometry.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab27321 被引用在 3 文献中.
- Zhong L et al. miR-451a suppression of IL-6R can inhibit proliferation and increase apoptosis through the JAK2/STAT3 pathway in multiple myeloma. Oncol Lett 20:339 (2020). PubMed: 33123250
- Zhong L et al. Circulating miR-451a levels as a potential biomarker to predict the prognosis of patients with multiple myeloma. Oncol Lett 20:263 (2020). PubMed: 32989397
- Salem DA et al. Expression of the IL-6 receptor alpha-chain (CD126) in normal and abnormal plasma cells in monoclonal gammopathy of undetermined significance and smoldering myeloma. Leuk Lymphoma 59:178-186 (2018). PubMed: 28540748