FITC荧光抗人IgG抗体(ab81051)
Key features and details
- FITC Goat polyclonal to Human IgG
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
概述
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产品名称
FITC荧光抗人IgG抗体
参阅全部 Human IgG 一抗 -
描述
FITC荧光山羊多克隆抗体to人IgG -
宿主
Goat -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
Low background fluorescence of normal tissue in contrast to cases of autoimmune disease, deposited immunoglobulin IgG. No cross reactivity with albumin fractions of human serum. No cross reactivity with albumin fractions of animal species. -
经测试应用
适用于: IHC-Fr, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Human IgG.
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常规说明
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel filtation and ion exchange chromatography to clear unbound conjugate. F/P ratio: 4-6 mol Fluorescein per 1 mol of goat IgG.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.50
Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorbed by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81051于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
细胞定位
Cell Membrane and Secreted -
数据库链接
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
see all -
别名
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab81051 被引用在 4 文献中.
- Zhang C et al. Novel EGFR-bispecific recombinant immunotoxin based on cucurmosin shows potent anti-tumor efficiency in vitro. Oncol Rep N/A:N/A (2020). PubMed: 33416157
- Zhang C et al. Novel cucurmosin-based immunotoxin targeting programmed cell death 1-ligand 1 with high potency against human tumor in vitro and in vivo. Cancer Sci 111:3184-3194 (2020). PubMed: 32589330
- Heo W et al. STIM1 knock-down decreases the affinity of obinutuzumab for CD20 by altering CD20 localization to Triton-soluble membrane. Clin Exp Immunol 200:260-271 (2020). PubMed: 32056202
- Gramlich OW et al. Enhanced insight into the autoimmune component of glaucoma: IgG autoantibody accumulation and pro-inflammatory conditions in human glaucomatous retina. PLoS One 8:e57557 (2013). ICC/IF ; Human . PubMed: 23451242