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FITC荧光Anti-HLA A2抗体[BB7.2] (ab27728)

Submit a review Q&A (2)References (8)
Flow Cytometry - FITC Anti-HLA A2 antibody [BB7.2] (ab27728)

    Key features and details

    • FITC Mouse monoclonal [BB7.2] to HLA A2
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2b

    Conjugates logo Related conjugates and formulations

    Carrier Free

    概述

    • 产品名称

      FITC荧光Anti-HLA A2抗体[BB7.2]
      参阅全部 HLA A2 一抗

    • 描述

      FITC荧光小鼠单克隆抗体[BB7.2] to HLA A2

    • 宿主

      Mouse

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    • 特异性

      Ab27728 recognises the human HLA A2 histocompatability antigen.

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Full length protein corresponding to Human HLA A2. Papain solubilized HLA-A2

    • 表位

      The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy terminus of the alpha 2 helix and a turn on one of the underlying beta strands.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      Preservative: 0.09% Sodium azide
      Constituent: 1% BSA
    • Concentration information loading...

    • 纯度

      Protein G purified

    • 纯化说明

      Ab27728 was purified from tissue culture supernatant, and prepared by affinity chromatography.

    • 克隆

      单克隆

    • 克隆编号

      BB7.2

    • 骨髓瘤

      NS1

    • 同种型

      IgG2b

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab27728于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    1/10.

    Use 10 µl of the suggested working dilution to label 106 cells or 100µl whole blood.

     

     

     

    ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     
    说明
    Flow Cyt
    1/10.

    Use 10 µl of the suggested working dilution to label 106 cells or 100µl whole blood.

     

     

     

    ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     

    靶标

    • 相关性

      Involved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein.

    • 细胞定位

      Membrane; Single-pass type I membrane protein.

    • 数据库链接

      • 别名

        • Antigen presenting molecule antibody
        • HLA A antibody
        • HLA A histocompatibility type antibody
        • HLA antibody
        • HLA class I antibody
        • HLA class I histocompatibility antigen A 2 alpha chain antibody
        • HLA class I histocompatibility antigen A2 alpha chain antibody
        • HLA-A antibody
        • HLAA antibody
        • Human leukocyte antigen antibody
        • Leucocyte antigen class I antibody
        • Leukocyte antigen class I A antibody
        • Major histocompatibility complex class I A antibody
        • Major histocompatibility complex class I antibody
        • MHC class I antigen [precursor] antibody
        • MHC class I antigen A antibody
        • MHC class I antigen A*2 antibody
        • MHC class I antigen A2 antibody
        • MHC class I antigen HLA A antibody
        • MHC class I antigen HLA A heavy chain antibody
        • MHC leukocyte antigen antibody
        see all

      图片

      • Flow Cytometry - FITC Anti-HLA A2 antibody [BB7.2] (ab27728)
        Flow Cytometry - FITC Anti-HLA A2 antibody [BB7.2] (ab27728)
        Ab27728, undiluted or at a dilution of 1/10, staining HLA A2 in human peripheral blood monocytes by FACS.

      文献 (8)

      发表研究结果有使用 ab27728？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab27728 被引用在 8 文献中.

      • Viborg N  et al. T cell recognition of novel shared breast cancer antigens is frequently observed in peripheral blood of breast cancer patients. Oncoimmunology 8:e1663107 (2019). PubMed: 31741759
      • Li D  et al. Engineering chimeric human and mouse major histocompatibility complex (MHC) class I tetramers for the production of T-cell receptor (TCR) mimic antibodies. PLoS One 12:e0176642 (2017). Flow Cyt . PubMed: 28448627
      • Kim YH  et al. Branched multipeptide immunotherapy for glioblastoma using human leukocyte antigen-A*0201-restricted cytotoxic T-lymphocyte epitopes from ERBB2, BIRC5 and CD99. Oncotarget 7:50535-50547 (2016). PubMed: 27409668
      • Straetemans T  et al. Recurrence of melanoma following T cell treatment: continued antigen expression in a tumor that evades T cell recruitment. Mol Ther 23:396-406 (2015). PubMed: 25363716
      • Cheent KS  et al. Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94. Proc Natl Acad Sci U S A 110:16981-6 (2013). Human . PubMed: 24082146
      • Whitfield-Larry F  et al. HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/?c(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells. Diabetes 60:1726-33 (2011). Flow Cyt . PubMed: 21521873
      • Hogan KT & Brown SL Localization and characterization of serologic epitopes on HLA-A2. Hum Immunol 33:185-92 (1992). PubMed: 1377666
      • Parham P & Brodsky FM Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum Immunol 3:277-99 (1981). PubMed: 7035415

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Question

      The Ab reported into this paper is not ab74674 but the FITC conjugated ab27728.
      Kind regards

      Read More
      Answer

      Thank you for contacting us.

      Unfortunately we have not determined which specific HLA-A2 allele(s) this clone binds. However, it is clear from the paper you kindly provided that the clone binds HLA-A201. Unfortunately, we currently do not have any empirical data to indicate if this clone is specific just to the HLA-A201 allele or if detects other alleles.

      http://www.ncbi.nlm.nih.gov/pubmed/19265143

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      BATCH NUMBER 217168 ORDER NUMBER 168928 DESCRIPTION OF THE PROBLEM No signal. SAMPLE (Human) SupT1 cells (Human) Jurkat T cells PRIMARY ANTIBODY AbCam: Mouse monoclonal [BB7.2] to HLA A2(FITC). Used neat at 10uL per million cells Incubated for 1hr on ice or 1hr at Room Temp. Washed either once, twice or three times with 1xPBS DETECTION METHOD FACS POSITIVE AND NEGATIVE CONTROLS USED Negative Controls: Used unstained cells from both cell lines. Positive Controls: Used MHC-APC antibody* Initially used MHC-APC antibody*, but was hoping to use the AbCam to isolate specifically the HLA A2 population. ANTIBODY STORAGE CONDITIONS Ab received at room temp. Kept at 4C for ~2.5 weeks (while being tested) remainder was aliquoted and kept at -20C. SAMPLE PREPARATION Both cell lines are grown in 10% RPMI Ab/Amyc. Cells were resuspended in either solution buffers prior to Ab staining: 1xPBS or 1xPBS + 2%FBS NUMBER OF CELLS USED 0.5x10^6 (500,000) 1.0x10^6 (1,000,000) PERMEABILIZATION STEP N/A BLOCKING CONDITIONS Either not blocked (1xPBS) or blocked (1xPBS + 2%FBS) ~20min SECONDARY ANTIBODY N/A HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Altered primary AbCam conjugated Ab incubation times (45 min up to 1.25 hours), whether on ice or not, and whether (or not) blocking (FBS) solution was included.

      Read More
      Answer

      Thank you for filling out the questionnaire. Your protocol looks good. Do you incubate the cells with the antibody in the dark? FITC is sensitive to photo-bleaching. The only other recommendation I can make would be to try BSA instead of FBS for blocking, in case there are interactions with fetal serum proteins other than BSA that are blocking binding of the antibody to the HLA A2. The PBS-only control is a good idea but you may be losing the antibody to hydrophobic interactions with the tube you incubate in when you leave out blocking protein. The BSA is admittedly a long-shot solution. If you feel photo-bleaching is not a problem, then I can send you a free-of-charge replacement vial, or a refund or credit. Let me know what you would like.

      Read More

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