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Microbiology Organism Virus DNA Virus double stranded DNA Virus Hepatitis B

FITC荧光抗乙型肝炎病毒Surface Antigen (Ad/Ay)抗体(ab20930)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (1)

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Key features and details

  • FITC Goat polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay)
  • Suitable for: ELISA
  • Reacts with: Hepatitis B virus
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

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概述

  • 产品名称

    FITC荧光抗乙型肝炎病毒Surface Antigen (Ad/Ay)抗体
    参阅全部 Hepatitis B Virus Surface Antigen (Ad/Ay) 一抗
  • 描述

    FITC荧光山羊多克隆抗体to乙型肝炎病毒Surface Antigen (Ad/Ay)
  • 宿主

    Goat
  • 偶联物

    FITC. Ex: 493nm, Em: 528nm
  • 特异性

    Purified surface antigen. Monospecific, reacts only with Hepatitis B virus.
  • 经测试应用

    适用于: ELISAmore details
  • 种属反应性

    与反应: Hepatitis B virus
  • 免疫原

    Tissue, cells or virus corresponding to Hepatitis B virus Hepatitis B Virus Surface Antigen (Ad/Ay).

  • 常规说明

    Covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Hepatitis B
    • Cancer
    • Oncoproteins/suppressors
    • Viral proteins
    • Hepatitis B

相关产品

  • Isotype control

    • FITC Goat IgG - Isotype control (ab37374)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab20930于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.

靶标

  • 相关性

    Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
  • 细胞定位

    Virion membrane
  • 数据库链接

    • Entrez Gene: 2828293 Hepatitis B virus
    • Entrez Gene: 944569 Hepatitis B virus
    • SwissProt: P03138 Hepatitis B virus
    • SwissProt: P03140 Hepatitis B virus
    • SwissProt: P03141 Hepatitis B virus
    • SwissProt: Q81157 Hepatitis B virus
    • SwissProt: Q81158 Hepatitis B virus
    • SwissProt: Q81159 Hepatitis B virus
    • SwissProt: Q81160 Hepatitis B virus
    see all
  • 别名

    • HBsAg antibody
    • HBV major surface antigen antibody
    • HBV surface antigen antibody
    • Hepatitis B Virus major surface antigen antibody
    • Major surface antigen antibody
    • S antibody
    see all

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (1)

发表研究结果有使用 ab20930?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab20930 被引用在 1 文献中.

  • Ortega-Prieto AM  et al. 3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection. Nat Commun 9:682 (2018). PubMed: 29445209

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Flow Cytometry abreview for Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (FITC)

Inconclusive
Abreviews
Abreviews
Application
Flow Cytometry
Sample
Human Cell (peripheral immune cells)
Permeabilization
No
Gating Strategy
w work on purified cells. pDC were gated on BDCA2+HLADR+ and then
Specification
peripheral immune cells
Preparation
Cell harvesting/tissue preparation method: isolation of PBMC after ficoll plaque and purification of plasmacytoid dendritic cells
Sample buffer: cells were cultured in RPMI 10% SVF with the viral Ag at 4°C or 37°C and then wash before incubation with this anti-body.
Fixation
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 27 2016

Question

same as ab20930 but UNCONJUGATED.

Read More

Abcam community

Verified customer

Asked on Sep 12 2012

Answer

Thank you for your inquiry.

Please have follow this link for all our Hepatitis B Virus Surface Antigen (Ad/Ay) antibodies that are un-conjugated:

https://www.abcam.com/index.html?pageconfig=searchresults&search=Hepatitis B Virus Surface Antigen Ad Ay&pt=1&sk=targ&sv=Hepatitis B Virus Surface Antigen ad %2B ay&sn=Hepatitis B Virus Surface Antigen ad %2B ay&l=3&fViewMore=1

I will be happy to provide further help in choosing a suitable antibody when you provide more information about the samples, application etc you are planning to use.

Read More

Abcam Scientific Support

回复于 Sep 12 2012

Question

I have been using this antibody in Flow Cytometry. I have sucessfully stained infected cells, however I am detecting positive signal in healthy control cells as well. I have tested multiple fixation methods as well as antibody dilutions of 1:10 - 1:100. I would like to try another lot of antibody.

Read More

Abcam community

Verified customer

Asked on Apr 17 2012

Answer

This is to confirm that a vial of ab20930 has been added free of charge to Cedarlane's order and will be dispatched today.

Read More

Abcam Scientific Support

回复于 Apr 17 2012

Question

Dear Sir/Madam, May I enquire if there is any isotype control for the above mention antibody (AB20930) ? Many thanks for your assistance and hope to hear from you soon. Best Regards,

Read More

Abcam community

Verified customer

Asked on Sep 02 2011

Answer

Thank you for your enquiry. I can confirm that ab20930 Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody is a goat polyclonal antibody. Is is very unusual to have isotype controls for polyclonal antibodies, becuase they contain a mixture of isotypes. Therefore, regrettably we do not have a goat polyclonal isotype control available in the catalogue. I am sorry there are no suitable isotype controls to meet your requirements on this occasion. Should you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

回复于 Sep 02 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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