FITC荧光Anti-Glycophorin A抗体[YTH89.1] (ab28082)
![Flow Cytometry - FITC Anti-Glycophorin A antibody [YTH89.1] (ab28082)](https://www.abcam.cn/ps/products/28/ab28082/Images/ab28082-38973-anti-glycophorin-a-antibody-yth891-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-Glycophorin A antibody [YTH89.1] (ab28082)")
Key features and details
- FITC Rat monoclonal [YTH89.1] to Glycophorin A
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-Glycophorin A抗体[YTH89.1]
参阅全部 Glycophorin A 一抗 -
描述
FITC荧光大鼠单克隆抗体[YTH89.1] to Glycophorin A -
宿主
Rat -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Human red blood cells followed by purified glycophorin A.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Ammonium Sulphate Precipitation -
纯化说明
Ig fraction prepared by ammonium sulphate precipitation -
克隆
单克隆 -
克隆编号
YTH89.1 -
骨髓瘤
Y3/Ag1.2.3 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28082于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
| 说明 |
|---|
应用说明
Flow Cyt: Use neat.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). -
序列相似性
Belongs to the glycophorin A family. -
翻译后修饰
The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific). -
细胞定位
Cell membrane. Appears to be colocalized with SLC4A1. - Information by UniProt
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数据库链接
- Entrez Gene: 2993 Human
- Omim: 111300 Human
- SwissProt: P02724 Human
- Unigene: 434973 Human
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别名
- AI853584 antibody
- Blood group--MN locus antibody
- CD_antigen=CD235a antibody
see all
图片
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Flow Cytometry - FITC Anti-Glycophorin A antibody [YTH89.1] (ab28082)Staining of human peripheral blood erythrocytes with neat rat anti human CD235a:FITC (ab28082)
数据表及文件
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Datasheet download
文献 (3)
ab28082 被引用在 3 文献中.
- Emmerson O et al. The Impact of Two Combined Oral Contraceptives Containing Ethinyl Estradiol and Drospirenone on Whole Blood Clot Viscoelasticity and the Biophysical and Biochemical Characteristics of Erythrocytes. Microsc Microanal 24:713-728 (2018). PubMed: 30588913
- Nanayakkara NP et al. Scalable preparation and differential pharmacologic and toxicologic profiles of primaquine enantiomers. Antimicrob Agents Chemother 58:4737-44 (2014). Flow Cyt ; Mouse . PubMed: 24913163
- Jokiranta TS & Meri S Biotinylation of monoclonal antibodies prevents their ability to activate the classical pathway of complement. J Immunol 151:2124-31 (1993). PubMed: 7688394
