FITC荧光Anti-Glycophorin A + B抗体[HIR2] (ab52452)
![Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)](https://www.abcam.cn/ps/products/52/ab52452/Images/ab52452-1-fitc-anti-glycophorin-a-b-antibody-hir2-flow-cytometry-peripheral-blood-human.jpg "Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)")
Key features and details
- FITC Mouse monoclonal [HIR2] to Glycophorin A + B
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-Glycophorin A + B抗体[HIR2]
参阅全部 Glycophorin A + B 一抗 -
描述
FITC荧光小鼠单克隆抗体[HIR2] to Glycophorin A + B -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
ab52452 recognises N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins expressed on red blood cells and erythroid precursor cells. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).
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阳性对照
- Flow Cyt: A subset of erythrocytes in human peripheral blood.
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常规说明
ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Prior to conjugation, ab52452 was purified by Protein G affinity chromatography. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE. -
Primary antibody说明
ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). -
克隆
单克隆 -
克隆编号
HIR2 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52452于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
靶标
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相关性
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). -
细胞定位
Type I membrane protein. -
数据库链接
- Entrez Gene: 2993 Human
- Entrez Gene: 2994 Human
- SwissProt: P02724 Human
- SwissProt: P06028 Human
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别名
- CD235a antibody
- CD235b antibody
- Erythroid lineage specific membrane sialoglycoprotein antibody
see all
图片
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Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)Flow cytometry analysis (surface staining) of human peripheral blood labeling CD235a with ab52452.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab52452 尚未被引用在任何文献中。