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Tags & Cell Markers Fusion / Marker Proteins GFP

FITC荧光Anti-GFP抗体(ab6662)

  • Datasheet
  • SDS
Reviews (14)Q&A (7)References (171)

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Western blot - FITC Anti-GFP antibody (ab6662)
  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
  • IHC - Wholemount - FITC Anti-GFP antibody (ab6662)
  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)

Key features and details

  • FITC Goat polyclonal to GFP
  • Suitable for: IHC-FoFr, IHC-Fr, WB, ICC/IF
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Alexa Fluor® 488 Anti-GFP antibody [EPR14104] - GFP booster (ab225314)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    FITC荧光Anti-GFP抗体
    参阅全部 GFP 一抗
  • 描述

    FITC荧光山羊多克隆抗体to GFP
  • 宿主

    Goat
  • 偶联物

    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用

    适用于: IHC-FoFr, IHC-Fr, WB, ICC/IFmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Recombinant full length protein corresponding to GFP aa 1-246.
    Sequence:

    MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT GKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFF KDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNV YIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY LSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK


    Database link: P42212
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: Recombinant A. victoria GFP protein.
  • 常规说明

    Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.
    In case of unexpected background, use pre-adsorbed secondary antibodies.

    Fluorescein isothiocyanate (FITC) (MW 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.5 moles FITC per mole of Goat IgG

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 6.50
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA Immunoglobulin and Protease free
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 纯化说明

    GFP Fluorescein Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Primary antibody说明

    Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GFP

相关产品

  • Isotype control

    • FITC Goat IgG - Isotype control (ab37374)
  • Recombinant Protein

    • Recombinant E. coli GFP protein (His tag) (ab119740)
    • Recombinant A. victoria GFP protein (ab84191)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • GFP ELISA Kit (ab171581)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6662于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-FoFr (1)
1/250.
IHC-Fr (5)
Use at an assay dependent concentration.
WB (1)
1/10000 - 1/100000.
ICC/IF (4)
1/200 - 1/400.
说明
IHC-FoFr
1/250.
IHC-Fr
Use at an assay dependent concentration.
WB
1/10000 - 1/100000.
ICC/IF
1/200 - 1/400.

靶标

  • 相关性

    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • 别名

    • GFP antibody
    • Green fluorescent protein antibody

图片

  • Western blot - FITC Anti-GFP antibody (ab6662)
    Western blot - FITC Anti-GFP antibody (ab6662)
    FITC Anti-GFP antibody (ab6662) + Recombinant A. victoria GFP protein (ab84191)

    Secondary
    Fluorescein goat secondary antibody for 60 minutes at RT at 1/1000 dilution


    Block: Blocking buffer for 30 minutes at RT.

  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
    Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)Kim at el PLoS Genet. 2018 Feb 8;14(2):e1007204. doi: 10.1371/journal.pgen.1007204. eCollection 2018 Feb. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Oscillation of Cyclin E and E2F target gene expression is deregulated in de2f1b mutant salivary glands.

    Salivary glands of control and de2f1b mutant early (80–85 hr AEL) third instar larvae expressing PCNA-GFP (green, ab6662) are stained with anti-dE2F1 (red). The region where high PCNA-GFP is observed with low dE2F1 is marked by an asterisk.

    For Immunostaining, third instar imaginal discs and salivary glands were dissected in PBS and immediately fixed in 4% formaldehyde in PBS for 20 minutes at room temperature with the exception of tissues subjected to anti-dE2F1 staining that were fixed for 30 minutes on ice. Fixed tissues were then washed with 0.3% PBST (0.3% TritonX-100 in 1XPBS) and 0.1% PBST (0.1% TritonX-100 in 1XPBS). Samples were incubated with appropriate amount of primary antibody in 0.1% PBST and 1%BSA overnight. Samples were then washed with 0.1% PBST, incubated in secondary antibody in 0.1% PBST and 1% BSA for 2 hours, followed by several washes in 0.1% PBST prior to mounting.

  • IHC - Wholemount - FITC Anti-GFP antibody (ab6662)
    IHC - Wholemount - FITC Anti-GFP antibody (ab6662)

    Immunofluorescence Microscopy using ab6662.

    Tissue: Drosophila melanogaster late stage embryonic central nervous system.

    Fixation: 0.5% PFA.

    Antigen retrieval: Not required.

    Primary antibody: Anti-GFP antibody at a 1/1,000 for 1 h at RT.

    Secondary antibody: AlexaFluor 488™ conjugated anti-Goat antibody at 1/300 for 45 minutes at RT.

    Panel A: shows a lateral view (ventral left).

    Panels B and C: shows ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom).

    In all panels, anterior is up.

    Staining: tau-GFP cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) as green fluorescent signal.

  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
    Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)This image is courtesy of an Abreview submitted by Dr Joshua Breunig

    ab6662 staining mouse brain tissue sections (inducible GFP reporter) by IHC-Fr.

    The tissue was paraformaldehyde fixed and blocked with serum and then incubated with the antibody at a 1/1000 dilution for 1 hour.

    Staining is shown in the left hand panel. The middle panel shows staining with a rabbit anti-GFP antibody and the right hand panel shows the merged images (plus DAPI).

    ab6662 gives no noticable background and it is found that when viewing on an epifluorescent the exposure time is significantly reduced.

    See Abreview

  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
    Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)

    Immunofluorescence Microscopy using ab6662.

    Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification).

    Fixation: 4% PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution.

    Antigen retrieval: Frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns.

    Goat anti-GFP was used at 1/500 dilution in free floating imunnohistochemistry to detect GFP.

    Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1/10,000 for 45 minutes at RT.

    Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus.

    Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
    Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)

    These pictures show confocal immunofluorescence using GFP-expressing glial cells (green) transplanted into the lesioned rat spinal cord.

    Detected using ab6662 and a standard FITC filter set. Axons are labeled red by an antibody to neurofilament-200 and a rhodamine secondary antibody. The upper panel shows the centre of the transplant site at low power. Numerous GFP-positive cells can be seen mingling with axons. The lower panel shows, at high power in a single optical section, how ab6662 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious - the cell depicted can be seen wrapping around a neurofilament-200 positive axon.

    These images were kindly supplied as part of the review submitted by Andrew Toft.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (171)

发表研究结果有使用 ab6662?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6662 被引用在 171 文献中.

  • Ríos-Barrera LD & Leptin M An endosome-associated actin network involved in directed apical plasma membrane growth. J Cell Biol 221:N/A (2022). PubMed: 35061016
  • Meijer M  et al. Epigenomic priming of immune genes implicates oligodendroglia in multiple sclerosis susceptibility. Neuron 110:1193-1210.e13 (2022). PubMed: 35093191
  • Rodríguez-Urgellés E  et al. Meridianins Rescue Cognitive Deficits, Spine Density and Neuroinflammation in the 5xFAD Model of Alzheimer's Disease. Front Pharmacol 13:791666 (2022). PubMed: 35281935
  • Marcogliese PC  et al. Drosophila functional screening of de novo variants in autism uncovers damaging variants and facilitates discovery of rare neurodevelopmental diseases. Cell Rep 38:110517 (2022). PubMed: 35294868
  • Chen B  et al. Identification of macrophages in normal and injured mouse tissues using reporter lines and antibodies. Sci Rep 12:4542 (2022). PubMed: 35296717
View all Publications for this product

客户评价及客户问答

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1-10 of 21 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-GFP antibody (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (bone marrow)
Permeabilization
No
Specification
bone marrow
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 16 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody (FITC)

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Epithelial cells)
Permeabilization
Yes - Triton 100
Specification
Epithelial cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tina Yuan

Verified customer

提交于 Sep 16 2019

Immunohistochemistry (Frozen sections) abreview for Anti-GFP antibody (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Bone)
Permeabilization
No
Specification
Bone
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 16 2019

Western blot abreview for Anti-GFP antibody (FITC)

Average
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - nuclear (Bone)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Bone
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tina Yuan

Verified customer

提交于 Aug 28 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody (FITC)

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Long bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Citric Acid Based
Permeabilization
Yes - Triton 100
Specification
Long bone
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tina Yuan

Verified customer

提交于 Aug 28 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Davidson
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 08 2019

Immunohistochemistry free floating abreview for Anti-GFP antibody (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain)
Specification
brain
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

James Bogenpohl

Verified customer

提交于 Jul 16 2018

Immunohistochemistry (Frozen sections) abreview for Anti-GFP antibody (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Intestine)
Permeabilization
No
Specification
Intestine
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 18 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody (FITC)

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 17 2012

Question


je travaille sur une nouvelle application en cytométrie de flux en
neurosciences. J'aurais besoin d'un anticorps anti-GFP (fait dans la
chèvre ou l'âne, mais pas dans le lapin, la souris ou le poulet) déjà
couplé à du FITC. J'ai vu que vous disposiez d'une référence (ab6662)
qui correspond à ces critères mais elle n'est pas validée en
cytométrie de flux, et je ne peux pas me permettre de commander un
anticorps sans aucune certitude sur son bon fonctionnement. Je ne
connais pas vos politiques commerciales, mais serait-il envisageable
de tester un petit aliquot (10µL devraient suffire pour valider
l'anticorps), puis si cela fonctionne, vous envoyer les histogrammes
de cytométrie et effectuer une commande pour réaliser mes expériences?
Bien cordialement,

Read More

Abcam community

Verified customer

Asked on Dec 12 2012

Answer

Ab6662 n’a effectivement pas encore été testé en cytométrie en flux et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester cet anticorps en cytométrie en flux, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, un avoir de la valeur de l'anticorps testé vous sera offert.

Étapes à suivre :

1. Nous informer que vous souhaitez tester l’anticorps en cytométrie en flux et nous vous enverrons un code promotionnel (valable 4 mois) à utiliser après obtention et envoi de vos résultats,

2. Acheter l'anticorps,

3. Nous envoyer vos résultats (positifs ou négatifs) obtenus sous forme d'Abreview (www.abcam.com/abreviews).

4. L'envoi de vos résultats activera le code et vous donnera un avoir de la valeur de l'anticorps testé.

J'espère que ces informations vous aideront. N'hésitez pas à me contacter si vous avez besoin de plus de renseignements.

Read More

Abcam Scientific Support

回复于 Dec 12 2012

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