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Tags & Cell Markers Fusion / Marker Proteins GFP

FITC荧光Anti-GFP抗体(ab6662)

  • Datasheet
  • SDS
Reviews (15)Q&A (7)References (187)

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Western blot - FITC Anti-GFP antibody (ab6662)
  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)

Key features and details

  • FITC Goat polyclonal to GFP
  • Suitable for: IHC-FoFr, IHC-Fr, WB, ICC/IF
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Alexa Fluor® 488 Anti-GFP antibody [EPR14104] - GFP booster (ab225314)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    FITC荧光Anti-GFP抗体
    参阅全部 GFP 一抗
  • 描述

    FITC荧光山羊多克隆抗体to GFP
  • 宿主

    Goat
  • 偶联物

    FITC. Ex: 493nm, Em: 528nm
  • 特异性

    No reaction was observed against Human, Mouse and Rat Serum Proteins.

  • 经测试应用

    适用于: IHC-FoFr, IHC-Fr, WB, ICC/IFmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Recombinant full length protein corresponding to GFP aa 1-246.
    Sequence:

    MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT GKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFF KDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNV YIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY LSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK


    Database link: P42212
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: Recombinant A. victoria GFP protein.
  • 常规说明

    Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.
    In case of unexpected background, use pre-adsorbed secondary antibodies.

    Fluorescein isothiocyanate (FITC) (MW 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.5 moles FITC per mole of Goat IgG

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride

    BSA Immunoglobulin and Protease free
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    GFP Fluorescein Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Primary antibody说明

    Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GFP

相关产品

  • Isotype control

    • FITC Goat IgG - Isotype control (ab37374)
  • Recombinant Protein

    • Recombinant E. coli GFP protein (His tag) (ab119740)
    • Recombinant A. victoria GFP protein (ab84191)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)
    • GFP ELISA Kit (ab171581)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6662于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-FoFr (1)
1/250.
IHC-Fr (5)
Use at an assay dependent concentration.
WB (1)
1/10000.
ICC/IF (4)
1/500 - 1/2500.
说明
IHC-FoFr
1/250.
IHC-Fr
Use at an assay dependent concentration.
WB
1/10000.
ICC/IF
1/500 - 1/2500.

靶标

  • 相关性

    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • 别名

    • GFP antibody
    • Green fluorescent protein antibody

图片

  • Western blot - FITC Anti-GFP antibody (ab6662)
    Western blot - FITC Anti-GFP antibody (ab6662)
    FITC Anti-GFP antibody (ab6662) + Recombinant A. victoria GFP protein (ab84191)

    Secondary
    Fluorescein goat secondary antibody for 60 minutes at RT at 1/1000 dilution


    Block: Blocking buffer for 30 minutes at RT.

  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
    Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)Kim at el PLoS Genet. 2018 Feb 8;14(2):e1007204. doi: 10.1371/journal.pgen.1007204. eCollection 2018 Feb. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Oscillation of Cyclin E and E2F target gene expression is deregulated in de2f1b mutant salivary glands.

    Salivary glands of control and de2f1b mutant early (80–85 hr AEL) third instar larvae expressing PCNA-GFP (green, ab6662) are stained with anti-dE2F1 (red). The region where high PCNA-GFP is observed with low dE2F1 is marked by an asterisk.

    For Immunostaining, third instar imaginal discs and salivary glands were dissected in PBS and immediately fixed in 4% formaldehyde in PBS for 20 minutes at room temperature with the exception of tissues subjected to anti-dE2F1 staining that were fixed for 30 minutes on ice. Fixed tissues were then washed with 0.3% PBST (0.3% TritonX-100 in 1XPBS) and 0.1% PBST (0.1% TritonX-100 in 1XPBS). Samples were incubated with appropriate amount of primary antibody in 0.1% PBST and 1%BSA overnight. Samples were then washed with 0.1% PBST, incubated in secondary antibody in 0.1% PBST and 1% BSA for 2 hours, followed by several washes in 0.1% PBST prior to mounting.

  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
    Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)This image is courtesy of an Abreview submitted by Dr Joshua Breunig

    ab6662 staining mouse brain tissue sections (inducible GFP reporter) by IHC-Fr.

    The tissue was paraformaldehyde fixed and blocked with serum and then incubated with the antibody at a 1/1000 dilution for 1 hour.

    Staining is shown in the left hand panel. The middle panel shows staining with a rabbit anti-GFP antibody and the right hand panel shows the merged images (plus DAPI).

    ab6662 gives no noticable background and it is found that when viewing on an epifluorescent the exposure time is significantly reduced.

    See Abreview

  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
    Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)

    Immunofluorescence Microscopy using ab6662.

    Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification).

    Fixation: 4% PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution.

    Antigen retrieval: Frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns.

    Goat anti-GFP was used at 1/500 dilution in free floating imunnohistochemistry to detect GFP.

    Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1/10,000 for 45 minutes at RT.

    Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus.

    Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

  • Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
    Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)

    These pictures show confocal immunofluorescence using GFP-expressing glial cells (green) transplanted into the lesioned rat spinal cord.

    Detected using ab6662 and a standard FITC filter set. Axons are labeled red by an antibody to neurofilament-200 and a rhodamine secondary antibody. The upper panel shows the centre of the transplant site at low power. Numerous GFP-positive cells can be seen mingling with axons. The lower panel shows, at high power in a single optical section, how ab6662 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious - the cell depicted can be seen wrapping around a neurofilament-200 positive axon.

    These images were kindly supplied as part of the review submitted by Andrew Toft.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (187)

发表研究结果有使用 ab6662?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6662 被引用在 187 文献中.

  • Sancho-Balsells A  et al. Cognitive and Emotional Symptoms Induced by Chronic Stress Are Regulated by EGR1 in a Subpopulation of Hippocampal Pyramidal Neurons. Int J Mol Sci 24:N/A (2023). PubMed: 36835243
  • Sandoval-Rodríguez R  et al. D1 and D2 neurons in the nucleus accumbens enable positive and negative control over sugar intake in mice. Cell Rep 42:112190 (2023). PubMed: 36857179
  • Guo Y  et al. Protocol for indirect and direct co-culture between human cancer cells and endothelial cells. STAR Protoc 4:102177 (2023). PubMed: 37086411
  • Sunadome K  et al. Directionality of developing skeletal muscles is set by mechanical forces. Nat Commun 14:3060 (2023). PubMed: 37244931
  • Dushime H  et al. Fetal Muse-based therapy prevents lethal radio-induced gastrointestinal syndrome by intestinal regeneration. Stem Cell Res Ther 14:201 (2023). PubMed: 37568164
View all Publications for this product

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Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFP antibody (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Commercially available blocking buffer as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

提交于 Jan 07 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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