FITC荧光Anti-GFP抗体

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (AB6662)

These pictures show confocal immunofluorescence using GFP-expressing glial cells (green) transplanted into the lesioned rat spinal cord.

Detected using ab6662 and a standard FITC filter set. Axons are labeled red by an antibody to neurofilament-200 and a rhodamine secondary antibody. The upper panel shows the centre of the transplant site at low power. Numerous GFP-positive cells can be seen mingling with axons. The lower panel shows, at high power in a single optical section, how ab6662 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious - the cell depicted can be seen wrapping around a neurofilament-200 positive axon.

These images were kindly supplied as part of the review submitted by Andrew Toft.

• IHC-Fr

AbReview6795****

Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (AB6662)

ab6662 staining mouse brain tissue sections (inducible GFP reporter) by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum and then incubated with the antibody at a 1/1000 dilution for 1 hour. Staining is shown in the left hand panel. The middle panel shows staining with a rabbit anti-GFP antibody and the right hand panel shows the merged images (plus DAPI). ab6662 gives no noticable background and it is found that when viewing on an epifluorescent the exposure time is significantly reduced.

This image is courtesy of an Abreview submitted by Dr Joshua Breunig

• IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (AB6662)

Oscillation of Cyclin E and E2F target gene expression is deregulated in de2f1b mutant salivary glands.

Salivary glands of control and de2f1b mutant early (80–85 hr AEL) third instar larvae expressing PCNA-GFP (green, ab6662) are stained with anti-dE2F1 (red). The region where high PCNA-GFP is observed with low dE2F1 is marked by an asterisk.

For Immunostaining, third instar imaginal discs and salivary glands were dissected in PBS and immediately fixed in 4% formaldehyde in PBS for 20 minutes at room temperature with the exception of tissues subjected to anti-dE2F1 staining that were fixed for 30 minutes on ice. Fixed tissues were then washed with 0.3% PBST (0.3% TritonX-100 in 1XPBS) and 0.1% PBST (0.1% TritonX-100 in 1XPBS). Samples were incubated with appropriate amount of primary antibody in 0.1% PBST and 1%BSA overnight. Samples were then washed with 0.1% PBST, incubated in secondary antibody in 0.1% PBST and 1% BSA for 2 hours, followed by several washes in 0.1% PBST prior to mounting.

Kim at el PLoS Genet. 2018 Feb 8;14(2):e1007204. doi: 10.1371/journal.pgen.1007204. eCollection 2018 Feb. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

Supplier Data

Western blot - FITC Anti-GFP antibody (AB6662)

Block : Blocking buffer for 30 minutes at RT.

All lanes:

Western blot - FITC Anti-GFP antibody (ab6662)

All lanes:

Western blot - Recombinant A. victoria GFP protein (Tag Free) (<a href='/products/proteins-peptides/recombinant-a-victoria-gfp-protein-ab84191'>ab84191</a>)

Secondary

All lanes:

Fluorescein goat secondary antibody for 60 minutes at RT at 1/1000 dilution

Predicted band size: 27 kDa

false

• Dot

Supplier Data

Dot Blot - FITC Anti-GFP antibody (AB6662)

Dot Blot of Fluorescein conjugated Goat anti-GFP. Antigen : GFP Load : Five point 3-fold serial dilution starting at 100ng (Dot 1). Primary antibody : GFP Antibody Fluorescein Conjugated Pre-absorbed (ab6662) at 1/1,000 for 1hr at RT.

Blocking : Rockland's Blocking Buffer for Fluorescent Western Blotting.

Imaged with FITC filter.

All lanes:

Dot Blot - FITC Anti-GFP antibody (ab6662) at 1/1000 dilution

All lanes:

GFP

false