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FITC荧光Anti-F4/80抗体[BM8] (ab60343)

Submit a review Q&A (2)References (42)
Immunocytochemistry/ Immunofluorescence - FITC Anti-F4/80 antibody [BM8] (ab60343)
  • Flow Cytometry - FITC Anti-F4/80 antibody [BM8] (ab60343)

Key features and details

  • FITC Rat monoclonal [BM8] to F4/80
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Biotin Unconjugated

概述

  • 产品名称

    FITC荧光Anti-F4/80抗体[BM8]
    参阅全部 F4/80 一抗

  • 描述

    FITC荧光大鼠单克隆抗体[BM8] to F4/80

  • 宿主

    Rat

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

  • 经测试应用

    适用于: Flow Cyt, ICC/IFmore details

  • 种属反应性

    与反应: Mouse

  • 免疫原

    Tissue, cells or virus corresponding to Mouse F4/80. BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.
    Database link: 13733

  • 阳性对照

    • Mouse macrophages and spleen cells.

  • 常规说明

    This antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. It is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at +4°C. Store In the Dark.

  • 存储溶液

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% Gelatin, 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • 纯度

    Affinity purified

  • Primary antibody说明

    This antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. It is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.

  • 克隆

    单克隆

  • 克隆编号

    BM8

  • 同种型

    IgG2a

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab60343于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
1/50 - 1/100.

ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 1 µg/ml.
说明
Flow Cyt
1/50 - 1/100.

ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 1 µg/ml.

靶标

  • 功能

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

  • 组织特异性

    Expression is restricted to eosinophils.

  • 序列相似性

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ADGRE1 antibody
      • Adhesion G protein coupled receptor E1 antibody
      • Adhesion G protein-coupled receptor E1 antibody
      • AGRE1_HUMAN antibody
      • Cell surface glycoprotein EMR1 antibody
      • Cell surface glycoprotein F4/80 antibody
      • DD7A5 7 antibody
      • Egf like module containing mucin like hormone receptor like 1 antibody
      • Egf like module containing mucin like hormone receptor like sequence 1 antibody
      • EGF like module receptor 1 antibody
      • EGF TM7 antibody
      • EGF-like module receptor 1 antibody
      • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
      • EGFTM7 antibody
      • EMR 1 antibody
      • EMR1 antibody
      • EMR1 hormone receptor antibody
      • Gpf480 antibody
      • Ly71 antibody
      • Lymphocyte antigen 71 antibody
      • TM7LN3 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-F4/80 antibody [BM8] (ab60343)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-F4/80 antibody [BM8] (ab60343)

      ICC/IF image of ab60343 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1µg/ml, FITC conjugated) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry - FITC Anti-F4/80 antibody [BM8] (ab60343)
      Flow Cytometry - FITC Anti-F4/80 antibody [BM8] (ab60343)
      Overlay histogram showing RAW 264.7 cells stained with ab60343 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1/100 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) with secondary antibody DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a minimal signal in RAW 264.7 cells fixed with methanol (5 min) used under the same conditions.

      Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    文献 (42)

    发表研究结果有使用 ab60343？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab60343 被引用在 42 文献中.

    • Cheong LY  et al. Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice. Nat Commun 14:1213 (2023). PubMed: 36869026
    • Hernandez JC  et al. LIN28 and histone H3K4 methylase induce TLR4 to generate tumor-initiating stem-like cells. iScience 26:106254 (2023). PubMed: 36949755
    • Shi L  et al. Engineered FGF19ΔKLB protects against intrahepatic cholestatic liver injury in ANIT-induced and Mdr2-/- mice model. BMC Biotechnol 23:43 (2023). PubMed: 37789318
    • Qi H  et al. Activation of BZW1 by CEBPB in macrophages promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress in MRL/lpr lupus-prone mice. Cell Mol Biol Lett 28:79 (2023). PubMed: 37828427
    • Wang D  et al. Sonodynamical reversion of immunosuppressive microenvironment in prostate cancer via engineered exosomes. Drug Deliv 29:702-713 (2022). PubMed: 35236203
    View all Publications for this product

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    Hello. I am hoping to buy a flow cytometry antibody that sees human F4/80 antigen on peripheral blood mononuclear cell in vitro derived macrophages. Will the antibody ab60343 or any other antibody available from your company see human F4/80 on this cell type?

    Read More
    Answer

    Thank you for contacting us.

    Antibody ab60343 is a well-characterized F4/80 clone, BM8. If the cells express F4/80, the antibody will detect it. For more references, see the datasheet for the unconjugated version, ab16911:

    Click here (or use the following: https://www.abcam.com/F480-antibody-BM8-ab16911.html).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I have Rat mAb to F4/80 (BM8) ab 60343-100.　I want to know more about my antibody. Lot number is 526242. Could you send me the expiry date for this product.
    I am planning to use this antibody for IHC-P mouse heart. I would love to know if there is special recommendation/protocol for its use in IHC-P.

    Read More
    Answer

    Thank you for your enquiry.

    We do not have expire dates for our products and you should be able to use the product without anydifficulty if kept at the recommend storage temperature for at least one year. Please rest assured that our "Abpromise" guarantees a free of charge replacement or refund should the product not work as expectedwithin 6 months of purchase. If you have purchased this product more than a year ago, I would suggest to still try and use it.

    Regarding IHC-P protocol, please refer to the datasheet application information.

    IHC-P: 1/50. (fixation in 10% neutral buffered formalin for 24 h; blocking with non-immunized goat serum; microwaved for 6 min in citrate buffer; splenic macrophages as positive control.)

    I have also attached our recommended IHC-P protocol link for your reference.

    https://www.abcam.com/index.html?pageconfig=resource&rid=11384

    I hope this information will beuseful and help resolve any uncertainties you may have over the expire date. If there isanything else thatI canhelpyou with, please do not hesitate to contact me.

    Have a nice day!

    Read More

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