AB110429

FITC荧光Anti-DMPO Nitrone Adduct抗体[N1664A]

Name: FITC Anti-DMPO Nitrone Adduct抗体[N1664A] (ab110429)| Abcam中文官网
Brand: Abcam Limited
SKU: AB110429
Price: 4606 CNY
Availability: InStock

FITC Anti-DMPO Nitrone Adduct antibody [N1664A]

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(1 Publication)

Anti-DMPO Nitrone Adduct antibody [N1664A] conjugated to FITC - suitable for ICC samples. Clone [N1664A] is a mouse monoclonal antibody.

查看别名

55 dimethyl 1 pyrroline N oxide nitrone adduct

不同偶联物与剂型 (1)

Unconjugated

Anti-DMPO Nitrone Adduct antibody [N1664A]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

N1664A

亚型

IgG1

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

应用

ICC

applications

特异性

ab110429 recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Chemical": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

pH: 7.2 Constituents: 0.87% Sodium chloride, 0.27% Tripotassium orthophosphate, 0.1% BSA

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The DMPO nitrone adduct also known as 55-Dimethyl-1-Pyrroline N-Oxide adduct plays an important role in trapping free radicals during biological reactions. As a spin trap DMPO captures highly reactive radicals like hydroxyl and superoxide radicals converting them to more stable radical adducts that can be detected by Electron Spin Resonance (ESR) spectroscopy. The mass of the DMPO nitrone adduct varies depending on the specific radical bound but the core structure of DMPO nitrone has a molecular weight of 113.15 g/mol. DMPO adducts are expressed in conditioned systems and are especially useful in detecting radical species in cellular environments.

Biological function summary

DMPO nitrone adduct formation is critical for investigating oxidative stress within cells. It does not form part of a complex but acts as an independent trap for free radicals. By stabilizing reactive radicals DMPO adducts help to provide a clearer picture of the radical landscape within biological systems. This insight is essential for understanding the balance between reactive oxygen species production and antioxidant defense mechanisms in various cell types and tissues.

Pathways

DMPO nitrone adduct formation links closely to pathways involving oxidative stress and redox signaling. In these pathways DMPO assists in monitoring dynamic changes and interactions between radicals and antioxidant proteins like superoxide dismutase (SOD) and catalase. These pathways are important for maintaining cellular health and preventing oxidative damage.

The use of DMPO nitrone adduct is significant for research on oxidative stress-related conditions. It is connected to diseases like Alzheimer's and cardiovascular disorders where oxidative stress and free radical damage play a well-documented role. The formation of DMPO adducts provides a method for assessing the extent of radical involvement offering insights into disease mechanisms. The interaction between DMPO and proteins such as amyloid-beta in Alzheimer's highlights the importance of free radical monitoring in understanding disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information DMPO Nitrone Adduct

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Arteriosclerosis, thrombosis, and vascular biology 39:200-211 PubMed30580565

2018

Expansive Vascular Remodeling and Increased Vascular Calcification Response to Cholecalciferol in a Murine Model of Obesity and Insulin Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Luciana S Carmo,Emmanuel A Burdmann,Melissa R Fessel,Youri E Almeida,Luciana A Pescatore,Elisangela Farias-Silva,Lionel F Gamarra,Gabriel H Lopes,Thiago P A Aloia,Marcel Liberman

View all publications

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FITC荧光Anti-DMPO Nitrone Adduct抗体[N1664A]