FITC荧光Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体(ab2492)
Key features and details
- FITC Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
概述
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产品名称
FITC荧光Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体
参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗 -
描述
FITC荧光兔多克隆抗体to DDDDK tag (Binds to FLAG® tag sequence) -
宿主
Rabbit -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence). Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.
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常规说明
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
The molar F/P ratio is 4.1.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.2% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
应用 | Ab评论 | 说明 |
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WB |
说明 |
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Optimal working dilutions should be determined for your particular assay conditions.
靶标
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相关性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
别名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab2492 被引用在 4 文献中.
- Gangoso E et al. Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion. Cell 184:2454-2470.e26 (2021). PubMed: 33857425
- Redinus K et al. An Hb-mediated circulating macrophage contributing to pulmonary vascular remodeling in sickle cell disease. JCI Insight 4:N/A (2019). PubMed: 31391342
- Inoue N et al. Sema7A/PlxnCl signaling triggers activity-dependent olfactory synapse formation. Nat Commun 9:1842 (2018). PubMed: 29743476
- Guo Q et al. TCN, an AKT inhibitor, exhibits potent antitumor activity and enhances radiosensitivity in hypoxic esophageal squamous cell carcinoma in vitro and in vivo. Oncol Lett 13:949-954 (2017). PubMed: 28356983