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AB2492

FITC荧光Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体

FITC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

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(5 Publications)

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody conjugated to FITC is a rabbit polyclonal antibody suitable for Western blot.

查看别名

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk, flag, flag tag

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

No

应用

WB

applications

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替代产品

Primary Antibodies

AB1162

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

Lab Essentials

Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB1162)

  • 4
  • 15
Primary Antibodies

AB49763

HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB49763)

  • 5
  • 8
Primary Antibodies

AB205606

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB205606)

  • 5
  • 2
Primary Antibodies

AB236777

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (AB236777)

  • 5
  • 1
Primary Antibodies

AB125243

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]

Lab Essentials

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (AB125243)

  • 0
  • 0
Primary Antibodies

AB18230

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01]

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230)

  • 4
  • 7

搭配使用产品

Primary Antibodies

AB72469

PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72469)

  • 5
  • 1
Primary Antibodies

AB206566

Anti-V5 tag antibody [SV5-P-K]

Recombinant

Indirect ELISA - Anti-V5 tag antibody [SV5-P-K] (AB206566)

  • 5
  • 1
Primary Antibodies

AB72569

SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72569)

  • 0
  • 0
Primary Antibodies

AB245893

Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB245893)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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产品详情

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

The molar F/P ratio is 4.1.

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
Preservative: 0.1% Sodium azide Constituents: 0.2% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information DDDDK tag (Binds to FLAG® tag sequence)
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Nature chemical biology 17:1057-1064 PubMed34168368

2021

Rapid generation of potent antibodies by autonomous hypermutation in yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Alon Wellner,Conor McMahon,Morgan S A Gilman,Jonathan R Clements,Sarah Clark,Kianna M Nguyen,Ming H Ho,Vincent J Hu,Jung-Eun Shin,Jared Feldman,Blake M Hauser,Timothy M Caradonna,Laura M Wingler,Aaron G Schmidt,Debora S Marks,Jonathan Abraham,Andrew C Kruse,Chang C Liu

Cell 184:2454-2470.e26 PubMed33857425

2021

Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Ester Gangoso,Benjamin Southgate,Leanne Bradley,Stefanie Rus,Felipe Galvez-Cancino,Niamh McGivern,Esra Güç,Chantriolnt-Andreas Kapourani,Adam Byron,Kirsty M Ferguson,Neza Alfazema,Gillian Morrison,Vivien Grant,Carla Blin,IengFong Sou,Maria Angeles Marques-Torrejon,Lucia Conde,Simona Parrinello,Javier Herrero,Stephan Beck,Sebastian Brandner,Paul M Brennan,Paul Bertone,Jeffrey W Pollard,Sergio A Quezada,Duncan Sproul,Margaret C Frame,Alan Serrels,Steven M Pollard

JCI insight 4: PubMed31391342

2019

An Hb-mediated circulating macrophage contributing to pulmonary vascular remodeling in sickle cell disease.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Redinus,Jin Hyen Baek,Ayla Yalamanoglu,Hye Kyung H Shin,Radu Moldova,Julie W Harral,Delaney Swindle,David Pak,Scott K Ferguson,Rachelle Nuss,Kathryn Hassell,Eva Nozik-Grayck,Andre F Palmer,Mehdi A Fini,Vijaya Karoor,Kurt R Stenmark,Paul W Buehler,David C Irwin

Nature communications 9:1842 PubMed29743476

2018

Sema7A/PlxnCl signaling triggers activity-dependent olfactory synapse formation.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuko Inoue,Hirofumi Nishizumi,Hiromi Naritsuka,Hiroshi Kiyonari,Hitoshi Sakano

Oncology letters 13:949-954 PubMed28356983

2017

TCN, an AKT inhibitor, exhibits potent antitumor activity and enhances radiosensitivity in hypoxic esophageal squamous cell carcinoma and .

Applications

Unspecified application

Species

Unspecified reactive species

Qing Guo,Jia He,Feng Shen,Wei Zhang,Xi Yang,Chi Zhang,Qu Zhang,Jun-Xing Huang,Zheng-Dong Wu,Xin-Chen Sun,Sheng-Bin Dai
View all publications
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