FITC荧光Anti-Cytokeratin 8抗体[C-43] (ab176533)
![Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 8 antibody [C-43] (ab176533)](https://www.abcam.cn/ps/products/176/ab176533/Images/ab176533-455676-anti-cytokeratin-8-antibody-flow-cytometry-human-peripheral-whole-blood.jpg "Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 8 antibody [C-43] (ab176533)")
Key features and details
- FITC Mouse monoclonal [C-43] to Cytokeratin 8
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
FITC荧光Anti-Cytokeratin 8抗体[C-43]
参阅全部 Cytokeratin 8 一抗 -
描述
FITC荧光小鼠单克隆抗体[C-43] to Cytokeratin 8 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Sheep, Rabbit, Cow, Pig
不与反应:
Mouse, Rat, Chicken, Hamster, Xenopus laevis -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 8. Cytoskeleton preparation from HeLa Human cervix carcinoma cell line.
Database link: P05787 -
阳性对照
- Flow Cyt: A431 cellular suspension.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.098% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Size exclusion -
克隆
单克隆 -
克隆编号
C-43 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176533于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 1 - 2 µg/ml.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 1 - 2 µg/ml. |
靶标
-
功能
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
组织特异性
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
疾病相关
Cirrhosis -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
细胞定位
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
-
数据库链接
- Entrez Gene: 281269 Cow
- Entrez Gene: 3856 Human
- Entrez Gene: 100152077 Pig
- Entrez Gene: 100348891 Rabbit
- Entrez Gene: 101110495 Sheep
- Omim: 148060 Human
- SwissProt: P05786 Cow
- SwissProt: P05787 Human
see all -
别名
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
图片
-
Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 8 antibody [C-43] (ab176533)
Flow cytometry intracellular staining pattern of human peripheral whole blood mixed with A431 cellular suspension stained using ab176533 (concentration in sample 9 μg/ml).
-
![Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 8 antibody [C-43] (ab176533)](https://www.abcam.cn/ps/products/176/ab176533/Images/ab176533-455679-anti-cytokeratin-8-antibody-flow-cytometry-A431-cellular-suspension.jpg)
Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 8 antibody [C-43] (ab176533)Separation of A431 cellular suspension (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood mixed with A431 cellular suspension stained using ab176533 (concentration in sample 9 μg/ml).
数据表及文件
-
Datasheet download
文献 (2)
ab176533 被引用在 2 文献中.
- Tompkins J et al. Engineering CpG island DNA methylation in pluripotent cells through synthetic CpG-free ssDNA insertion. Cell Rep Methods 3:100465 (2023). PubMed: 37323577
- Sun H et al. Single-cell transcriptome analysis indicates fatty acid metabolism-mediated metastasis and immunosuppression in male breast cancer. Nat Commun 14:5590 (2023). PubMed: 37696831
