FITC荧光Anti-Cytokeratin 18抗体[C-04] (ab52459)
![Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)](https://www.abcam.cn/ps/products/52/ab52459/Images/ab52459-64565-anti-cytokeratin-18-antibody-c-04-fitc-flow-cytometry.jpg "Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)")
Key features and details
- FITC Mouse monoclonal [C-04] to Cytokeratin 18
- Suitable for: ICC, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-Cytokeratin 18抗体[C-04]
参阅全部 Cytokeratin 18 一抗 -
描述
FITC荧光小鼠单克隆抗体[C-04] to Cytokeratin 18 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: ICC, Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mammals
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免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 18. Cytoskeleton preparation of epidermal carcinoma cell line A431
Database link: P05783 -
阳性对照
- ICC: HCT116 cells; Flow Cyt (Intra): HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Prior to conjugation, the antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
C-04 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52459于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 5 µg/ml.
|
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| Flow Cyt (Intra) |
Use a concentration of 1 - 2 µg/ml.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
ICC
Use a concentration of 5 µg/ml. |
|
Flow Cyt (Intra)
Use a concentration of 1 - 2 µg/ml. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
组织特异性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
疾病相关
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
细胞定位
Cytoplasm > perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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别名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
图片
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Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)
Overlay histogram showing intracellular staining of HeLa cells labeled with ab52459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52459, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (FITC) (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Immunocytochemistry - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)](https://www.abcam.cn/ps/products/52/ab52459/Images/ab52459-64564-anti-cytokeratin-18-antibody-c-04-fitc-immunofluorescence.jpg)
Immunocytochemistry - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)ICC/IF image of ab52459 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52459, 5µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)](https://www.abcam.cn/ps/products/52/ab52459/Images/ab52459-455388-fitc-anti-cytokeratin-18-antibody-c-04-flow-cytometry-intracellular-hela-human.jpg)
Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)Flow cytometry (Intracellular) analysis HeLa (human cervix carcinoma cell line) cells labeling Cytokeratin 18 with ab52459 (red). Overlay with Isotype mouse IgG1 control FITC antibody (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab52459 被引用在 14 文献中.
- Jie XX et al. Detection of circulating tumor cells and evaluation of epithelial-mesenchymal transition patterns of circulating tumor cells in ovarian cancer. Transl Cancer Res 11:2636-2646 (2022). PubMed: 36093536
- Sabet Z et al. Talazoparib Does Not Interact with ABCB1 Transporter or Cytochrome P450s, but Modulates Multidrug Resistance Mediated by ABCC1 and ABCG2: An in Vitro and Ex Vivo Study. Int J Mol Sci 23:N/A (2022). PubMed: 36430819
- Fang L et al. TMPRSS2-ERG promotes the initiation of prostate cancer by suppressing oncogene-induced senescence. Cancer Gene Ther 29:1463-1476 (2022). PubMed: 35393570
- Zhang Y et al. Encorafenib Acts as a Dual-Activity Chemosensitizer through Its Inhibitory Effect on ABCC1 Transporter In Vitro and Ex Vivo. Pharmaceutics 14:N/A (2022). PubMed: 36559089
- Bernardini C et al. Development of a Pig Mammary Epithelial Cell Culture Model as a Non-Clinical Tool for Studying Epithelial Barrier-A Contribution from the IMI-ConcePTION Project. Animals (Basel) 11:N/A (2021). PubMed: 34359140