FITC荧光Anti-Ctip2抗体[25B6] (ab123449)
![Immunocytochemistry/ Immunofluorescence - FITC Anti-Ctip2 antibody [25B6] (ab123449)](https://www.abcam.cn/ps/products/123/ab123449/Images/ab123449-477117-Project20210823-ab123449-AP6482601-5ug-Jurkat-MeOH-DS-image-JPEG.jpg "Immunocytochemistry/ Immunofluorescence - FITC Anti-Ctip2 antibody [25B6] (ab123449)")
Key features and details
- FITC Rat monoclonal [25B6] to Ctip2
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-Ctip2抗体[25B6]
参阅全部 Ctip2 一抗 -
描述
FITC荧光大鼠单克隆抗体[25B6] to Ctip2 -
宿主
Rat -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Zebrafish
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免疫原
Recombinant fragment corresponding to Human Ctip2 aa 1-150.
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阳性对照
- Jurkat cells.
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常规说明
Fluorochrome/Protein Ratio: at least 3 moles FITC per mole of IgG.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
纯度
Ammonium Sulphate Precipitation -
纯化说明
Purity is near homogeneity as judged by SDS-PAGE. ab123449 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
克隆
单克隆 -
克隆编号
25B6 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab123449于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.125 - 1 µg/ml.
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| Flow Cyt (Intra) |
Use a concentration of 0.125 - 1 µg/ml.
|
| 说明 |
|---|
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ICC/IF
Use a concentration of 0.125 - 1 µg/ml. |
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Flow Cyt (Intra)
Use a concentration of 0.125 - 1 µg/ml. |
靶标
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功能
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element. -
组织特异性
Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. -
序列相似性
Contains 6 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 64919 Human
- Entrez Gene: 58208 Mouse
- Omim: 606558 Human
- SwissProt: Q9C0K0 Human
- SwissProt: Q99PV8 Mouse
- Unigene: 709690 Human
- Unigene: 392694 Mouse
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别名
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - FITC Anti-Ctip2 antibody [25B6] (ab123449)
ab123449 staining CTIP2 in Jurkat cells. The cells were fixed with 80% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab123449 at 1/100 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Flow Cytometry (Intracellular) - FITC Anti-Ctip2 antibody [25B6] (ab123449)](https://www.abcam.cn/ps/products/123/ab123449/Images/ab123449-136695-anti-ctip2-antibody-25b6-fitc-flow-cytometry.gif)
Flow Cytometry (Intracellular) - FITC Anti-Ctip2 antibody [25B6] (ab123449)ab123449 at 0.125µg/ml staining Ctip2 in Jurkat cells by Flow Cytometry (red) or an equal amount of an FITC conjugated control antibody (black) in 3% BSA block.The Jurkat cells show a clear CTIP2 specific signal -
![Flow Cytometry (Intracellular) - FITC Anti-Ctip2 antibody [25B6] (ab123449)](https://www.abcam.cn/ps/products/123/ab123449/Images/ab123449-136694-anti-ctip2-antibody-25b6-fitc-flow-cytometry.gif)
Flow Cytometry (Intracellular) - FITC Anti-Ctip2 antibody [25B6] (ab123449)ab123449 at 0.125µg/ml staining Ctip2 in SH-SY5Y cells by Flow Cytometry (red) or an equal amount of an FITC conjugated control antibody (black) in 3% BSA block. The SH-SY5Y cells do not show significant CTIP2 signal over the negative control signal.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab123449 被引用在 3 文献中.
- Del Toro D et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845
- Zhong C et al. Differential Expression of the Transcription Factor GATA3 Specifies Lineage and Functions of Innate Lymphoid Cells. Immunity 52:83-95.e4 (2020). PubMed: 31882362
- Forouzanfar F et al. HIV-1 Vpr mediates the depletion of the cellular repressor CTIP2 to counteract viral gene silencing. Sci Rep 9:13154 (2019). PubMed: 31511615
