FITC荧光Anti-CD90 / Thy1抗体[MRC OX-7] (ab226)
Key features and details
- FITC Mouse monoclonal [MRC OX-7] to CD90 / Thy1
- Suitable for: Flow Cyt (Intra), ICC
- Reacts with: Rat
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-CD90 / Thy1抗体[MRC OX-7]
参阅全部 CD90 / Thy1 一抗 -
描述
FITC荧光小鼠单克隆抗体[MRC OX-7] to CD90 / Thy1 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. -
经测试应用
适用于: Flow Cyt (Intra), ICCmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Rabbit, Guinea pig -
免疫原
corresponding to CD90/ Thy1.
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阳性对照
- ICC: PC12 cells. Flow Cyt (Intra): PC12 cells.
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常规说明
The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.
Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant -
克隆
单克隆 -
克隆编号
MRC OX-7 -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
- HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)
- PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
- PE Anti-CD90 / Thy1 antibody [MRC OX-7] (ab33694)
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/500.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC |
1/100.
This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min). |
说明 |
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Flow Cyt (Intra)
1/500. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC
1/100. This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min). |
靶标
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功能
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 21838 Mouse
- Entrez Gene: 24832 Rat
- SwissProt: P01831 Mouse
- SwissProt: P01830 Rat
- Unigene: 3951 Mouse
- Unigene: 108198 Rat
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别名
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
图片
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ab226 staining CD90 / Thy1 in undifferentiated PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI(shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)
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ab226 staining CD90 / Thy1 in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Flow cytometry overlay histogram showing PC12 (undifferentiated) cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab226) (1x 106 in 100µl at 1µg/ml (1/500)) for 30 min at 22°C.
Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in PC12 (undifferentiated) cells fixed with 4 % formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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Flow cytometry overlay histogram showing PC12 (undifferentiated) cells stained with ab226 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab226) (1x 106 in 100µl at 1µg/ml (1/500)) for 30 min on ice.
Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
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Overlay histogram showing PC12 cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab226, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab226 被引用在 27 文献中.
- Zhao Y et al. Mesenchymal Stem Cells Ameliorate Fibrosis by Enhancing Autophagy via Inhibiting Galectin-3/Akt/mTOR Pathway and by Alleviating the EMT via Inhibiting Galectin-3/Akt/GSK3β/Snail Pathway in NRK-52E Fibrosis. Int J Stem Cells 16:52-65 (2023). PubMed: 35483714
- Nair RS et al. A porcine cholecystic extracellular matrix conductive scaffold for cardiac tissue repair. J Biomed Mater Res B Appl Biomater 110:2039-2049 (2022). PubMed: 35305082
- Zhao J et al. Bone Regeneration and Angiogenesis by Co-transplantation of Angiotensin II-Pretreated Mesenchymal Stem Cells and Endothelial Cells in Early Steroid-Induced Osteonecrosis of the Femoral Head. Cell Transplant 31:9636897221086965 (2022). PubMed: 35313737
- Meng Q et al. Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway. J Cell Mol Med 25:1867-1883 (2021). PubMed: 33417281
- Wu X et al. Investigation of the role of miR-221 in diabetic peripheral neuropathy and related molecular mechanisms. Adv Clin Exp Med 30:623-632 (2021). PubMed: 34018345