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AB323935

FITC Anti-CD68 antibody [EPR23917-164]

FITC Anti-CD68 antibody [EPR23917-164]

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Rabbit Recombinant Monoclonal CD68 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Mouse samples.

查看别名

CD68, Macrosialin, Cd68

2 Images
Flow Cytometry (Intracellular) - FITC Anti-CD68 antibody [EPR23917-164] (AB323935)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-CD68 antibody [EPR23917-164] (AB323935)

Flow cytometry staining of C57 BL/6 Mouse Bone Marrow Cell-derived Macrophages with ab323935 (right) or Rabbit IgG [EPR25A] FITC (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab323935 or Rabbit IgG [EPR25A] FITC (1x 106 in 100 μl at 0.2 μg/ml (1/2500)) for 30min at 22°C. The cells were simultaneously stained with F4/80.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

Flow Cytometry (Intracellular) - FITC Anti-CD68 antibody [EPR23917-164] (AB323935)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-CD68 antibody [EPR23917-164] (AB323935)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab323935 (right) or Rabbit IgG [EPR25A] FITC (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab323935 or Rabbit IgG [EPR25A] FITC (1x 106 in 100 μl at 0.2 μg/ml (1/2500)) for 30min at 22°C. The cells were simultaneously stained with F4/80.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

不同偶联物与剂型 (8)

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD68 antibody [EPR23917-164]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD68 antibody [EPR23917-164]

  • 660 APC

    APC Anti-CD68 antibody [EPR23917-164]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD68 antibody [EPR23917-164]

  • 578 PE

    PE Anti-CD68 antibody [EPR23917-164]

  • Unconjugated

    Anti-CD68 antibody [EPR23917-164]

  • Carrier free

    Anti-CD68 antibody [EPR23917-164] - BSA and Azide free

  • Carrier free

    Anti-CD68 antibody [EPR23917-164] – Chicken IgY (Chimeric) – BSA and Azide Free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23917-164

亚型

IgG

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

No

反应种属

Mouse

应用

Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Mouse unsuitable for IHC-Fr application

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
See full target information Cd68
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搭配使用产品

Assay Kits

AB287167

Human CD68 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD68 ELISA Kit (AB287167)

  • 0
  • 0

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