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Cardiovascular Blood Other

FITC荧光Anti-CD62L抗体[FITC.FMC46] (ab222)

  • Datasheet
  • SDS
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Flow Cytometry - FITC Anti-CD62L antibody [FITC.FMC46] (ab222)

    Key features and details

    • FITC Mouse monoclonal [FITC.FMC46] to CD62L
    • Suitable for: Flow Cyt
    • Reacts with: Human, Monkey
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2b

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    概述

    • 产品名称

      FITC荧光Anti-CD62L抗体[FITC.FMC46]
      参阅全部 CD62L 一抗
    • 描述

      FITC荧光小鼠单克隆抗体[FITC.FMC46] to CD62L
    • 宿主

      Mouse
    • 偶联物

      FITC. Ex: 493nm, Em: 528nm
    • 特异性

      FITC.FMC46 recognises the human CD62L cell surface antigen, a 150 kD glycoprotein also known as L-selectin.
    • 经测试应用

      适用于: Flow Cytmore details
    • 种属反应性

      与反应: Human, Monkey
    • 免疫原

      Tissue, cells or virus corresponding to Human CD62L. PHA stimulated lymphoblasts

    • 常规说明

      Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C.
    • 存储溶液

      pH: 7.20
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified IgG prepared by affinity chromotography on protein G from tissue culture supernatant.
    • 克隆

      单克隆
    • 克隆编号

      FITC.FMC46
    • 骨髓瘤

      NS1
    • 同种型

      IgG2b
    • 轻链类型

      unknown
    • 研究领域

      • Cardiovascular
      • Blood
      • Other
      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Neutrophil Lineage
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells

    相关产品

    • Isotype control

      • FITC Mouse IgG2b [PLPV219] - Isotype Control (ab91368)
    • Recombinant Protein

      • Recombinant Human CD62L protein (ab132972)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab222于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use 10µl for 106 cells.

    Use 10 µl to label 106 cells or 100 µl whole blood.

    ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

    说明
    Flow Cyt
    Use 10µl for 106 cells.

    Use 10 µl to label 106 cells or 100 µl whole blood.

    ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

    靶标

    • 功能

      Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
    • 组织特异性

      Expressed in B cell lines and T lymphocytes.
    • 序列相似性

      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 2 Sushi (CCP/SCR) domains.
    • 细胞定位

      Membrane.
    • Target information above from: UniProt accession P14151 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 6402 Human
      • Omim: 153240 Human
      • SwissProt: P14151 Human
      • Unigene: 728756 Human
      • 别名

        • A.11 antibody
        • AI528707 antibody
        • CD62 antigen ligand antibody
        • CD62 antigen-like family member L antibody
        • CD62L antibody
        • gp90-MEL antibody
        • IgA nephropathy, susceptibility to, included antibody
        • L Selectin antibody
        • L-selectin antibody
        • LAM-1 antibody
        • LAM1 antibody
        • LECAM1 antibody
        • LEU8 antibody
        • Leukocyte adhesion molecule 1 antibody
        • Leukocyte surface antigen Leu-8 antibody
        • Leukocyte-endothelial cell adhesion molecule 1 antibody
        • Lnhr antibody
        • LSEL antibody
        • Ly-22 antibody
        • Ly-m22 antibody
        • Lyam-1 antibody
        • LYAM1 antibody
        • LYAM1_HUMAN antibody
        • Lymph node homing receptor antibody
        • Lymphocyte adhesion molecule 1 antibody
        • Lymphocyte antigen 22 antibody
        • Lymphocyte surface MEL-14 antigen antibody
        • MEL-14 antibody
        • Pln homing receptor antibody
        • PLNHR antibody
        • Selectin L antibody
        • Selectin, lymphocyte antibody
        • SELL antibody
        • TQ1 antibody
        see all

      图片

      • Flow Cytometry - FITC Anti-CD62L antibody [FITC.FMC46] (ab222)
        Flow Cytometry - FITC Anti-CD62L antibody [FITC.FMC46] (ab222)
        ab183 staining CD62L (FITC) in human peripheral blood granulocytes by Flow Cytometry analysis.

      实验方案

      • Flow cytometry protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (1)

      发表研究结果有使用 ab222?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab222 被引用在 1 文献中.

      • Lin HT  et al. Characterization of pancreatic stem cells derived from adult human pancreas ducts by fluorescence activated cell sorting. World J Gastroenterol 12:4529-35 (2006). PubMed: 16874866

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Question

      Hi there, Does this antibody cross-react with the rabbit species? or do you have anti-rabbit cd62l?

      Read More

      Abcam community

      Verified customer

      Asked on Nov 08 2001

      Answer

      This antibody has been shown to cross react with the human and bovine protein, it has not yet been tested in rabbit. Our monoclonal, ab6631, has also been tested in the same species. At this time we do not have an antibody to CD62L (L-selectin) that has been tested in rabbit

      Read More

      Abcam Scientific Support

      回复于 Nov 09 2001

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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