AB24897

FITC荧光Anti-CD45R抗体[RA3-6B2]

Name: FITC Anti-CD45R抗体[RA3-6B2] (ab24897)| Abcam中文官网
Brand: Abcam Limited
SKU: AB24897
Price: 4955 CNY
Availability: InStock

FITC Anti-CD45R antibody [RA3-6B2]

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(2 Publications)

Rat Monoclonal CD45 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

查看别名

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images

Flow Cytometry - FITC Anti-CD45R antibody [RA3-6B2] (AB24897)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD45R antibody [RA3-6B2] (AB24897)

Flow cytometry of C57Bl/6 splenocytes labeling CD45R with ab24897 at 0.25 μg (solid line) or Rat IgG2a FITC isotype control at 0.25 μg (dashed line).

不同偶联物与剂型 (5)

Unconjugated

Anti-CD45R antibody [RA3-6B2]
785 APC/Cy7®

APC/Cy7® Anti-CD45R antibody [RA3-6B2]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD45R antibody [RA3-6B2]
704 redFluor™ 710

redFluor™ 710 Anti-CD45R antibody [RA3-6B2]
450 violetFluor™ 450

violetFluor™ 450 Anti-CD45R antibody [RA3-6B2]

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

RA3-6B2

亚型

IgG2a

轻链类型

kappa

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Mouse

应用

Flow Cyt

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/fitc-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18446'>ab18446</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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产品详情

In vivo administration of ab24897 has been shown to affect differentiation of both T and B cells in normal mice and reduce the level of anti-DNA antibodies and lymphadenopathies in MRL/lpr mice.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

Purified from TCS

存储溶液

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD45R also known as Leukocyte Common Antigen or LCA is a protein that plays a significant role in immune cell function. It is an isoform of the larger protein CD45 which includes several alternatively spliced variants. CD45R is a transmembrane protein tyrosine phosphatase with a mass ranging from 180 to 220 kDa depending on its isoform. This protein is primarily expressed on hematopoietic cells including T cells B cells and other leukocytes. It functions mechanically by modulating signaling thresholds essential for lymphocyte development and activation.

Biological function summary

CD45R influences signaling pathways critical for immune response regulation. It does not form a complex by itself but interacts with other membrane receptors and cytoplasmic kinases to mediate signal transduction. The protein adjusts the activity of Src family kinases through its phosphatase activity impacting cell-cell communication and responsive behavior to antigens. CD45R is involved in maintaining a functional immune surveillance system particularly ensuring the adequate responsiveness of lymphocytes.

Pathways

CD45R's phosphatase activity is integral to the T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways. This activity regulates Lck and Fyn kinases within these pathways contributing to proper lymphocyte activation and development. Any disruption in CD45R function can affect these pathways potentially impacting proteins like ZAP-70 and Syk which are essential for signal transduction downstream of the TCR and BCR respectively.

CD45R has been implicated in conditions such as severe combined immunodeficiency (SCID) and autoimmune diseases like multiple sclerosis. Its malfunction or aberrant expression can cause improper immune system activation or suppression. In autoimmune contexts CD45R's interaction with other proteins like CTLA-4 may become dysregulated leading to immune system attacks on the body's tissues. Understanding these connections helps in developing therapeutic strategies targeting immune dysfunctions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 33:108236 PubMed33027652

2020

Cystatin C Plays a Sex-Dependent Detrimental Role in Experimental Autoimmune Encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Vahid Hoghooghi,Alexandra L Palmer,Ariana Frederick,Yulan Jiang,Jessica E Merkens,Anjali Balakrishnan,Trisha M Finlay,Anders Grubb,Efrat Levy,Paul Gordon,Frank R Jirik,Minh Dang Nguyen,Carol Schuurmans,Frank Visser,Shannon E Dunn,Shalina S Ousman

European journal of microbiology & immunology 4:123-7 PubMed24883198

2014

Evaluation of immune responses by live infectious bursal disease vaccines to avoid vaccination failures.

Applications

Unspecified application

Species

Unspecified reactive species

P Jakka,Y K Reddy,J J Kirubaharan,N D J Chandran

View all publications

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FITC荧光Anti-CD45R抗体[RA3-6B2]