FITC荧光Anti-CD45抗体[B-A11] (ab27287)
![Flow Cytometry - FITC Anti-CD45 antibody [B-A11] (ab27287)](https://www.abcam.cn/ps/products/27/ab27287/Images/ab27287-38009-anti-cd45-antibody-b-a11-fitc-flow-cytometry.gif "Flow Cytometry - FITC Anti-CD45 antibody [B-A11] (ab27287)")
Key features and details
- FITC Mouse monoclonal [B-A11] to CD45
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
概述
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产品名称
FITC荧光Anti-CD45抗体[B-A11]
参阅全部 CD45 一抗 -
描述
FITC荧光小鼠单克隆抗体[B-A11] to CD45 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
Ab27287 recognises the Leucocyte Common Antigen (LCA) with different isoforms of 180-190-205-220 kDa.
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经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD45. Thymus cells and Jurkat cell line
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.30
Preservative: 0.09% Sodium azide
Constituents: 94.91% PBS, 5% BSA -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
克隆
单克隆 -
克隆编号
B-A11 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27287于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Use 10 µl to label 106 cells or 100 µl of whole blood. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Use 10 µl to label 106 cells or 100 µl of whole blood. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
靶标
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功能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
疾病相关
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
结构域
The first PTPase domain interacts with SKAP1. -
翻译后修饰
Heavily N- and O-glycosylated. -
细胞定位
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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别名
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
图片
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Flow Cytometry - FITC Anti-CD45 antibody [B-A11] (ab27287)A typical lymphocytes staining pattern with ab27287.
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab27287 被引用在 27 文献中.
- Finburgh EN et al. Role of FGF10/FGFR2b Signaling in Homeostasis and Regeneration of Adult Lacrimal Gland and Corneal Epithelium Proliferation. Invest Ophthalmol Vis Sci 64:21 (2023). PubMed: 36715672
- Xu K et al. Long non-coding RNA X-Inactive Specific Transcript (XIST) interacting with USF2 promotes osteogenic differentiation of periodontal ligament stem cells through regulation of WDR72 transcription. J Periodontal Res 58:1235-1247 (2023). PubMed: 37712743
- Ning T et al. BRD4 facilitates osteogenic differentiation of human bone marrow mesenchymal stem cells through WNT4/NF-κB pathway. J Orthop Surg Res 18:876 (2023). PubMed: 37980502
- Wang SJ et al. Bone marrow mesenchymal stem cell-derived extracellular vesicles containing miR-181d protect rats against renal fibrosis by inhibiting KLF6 and the NF-κB signaling pathway. Cell Death Dis 13:535 (2022). PubMed: 35672285
- Poursafavi Z et al. Differentiation of Alginate-Encapsulated Wharton Jelly-Derived Mesenchymal Stem Cells into Insulin Producing Cells. Cell J 24:449-457 (2022). PubMed: 36093804