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FITC荧光Anti-CD34抗体[4H11[APG]] (ab18227)

Submit a review Q&A (1)References (11)
Flow Cytometry - FITC Anti-CD34 antibody [4H11[APG]] (ab18227)

    Key features and details

    • FITC Mouse monoclonal [4H11[APG]] to CD34
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    概述

    • 产品名称

      FITC荧光Anti-CD34抗体[4H11[APG]]
      参阅全部 CD34 一抗

    • 描述

      FITC荧光小鼠单克隆抗体[4H11[APG]] to CD34

    • 宿主

      Mouse

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human CD34. Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia

    • 表位

      Class III epitope on CD34 antigen.

    • 阳性对照

      • Human blood cells.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab18227于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    说明
    应用说明
    Flow Cyt: Use 20 µl reagent / 100 µl sample.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    靶标

    • 功能

      Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

    • 组织特异性

      Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

    • 序列相似性

      Belongs to the CD34 family.

    • 发展阶段

      On early hematopoietic progenitor cells.

    • 翻译后修饰

      Highly glycosylated.
      Phosphorylated on serine residues by PKC.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD34 antibody
        • CD34 antigen antibody
        • CD34 molecule antibody
        • CD34_HUMAN antibody
        • Cluster designation 34 antibody
        • cluster of differentiation 34 antibody
        • Hematopoietic progenitor cell antigen CD34 antibody
        • HPCA1 antibody
        • Mucosialin antibody
        • OTTHUMP00000034733 antibody
        • OTTHUMP00000034734 antibody
        see all

      图片

      • Flow Cytometry - FITC Anti-CD34 antibody [4H11[APG]] (ab18227)
        Flow Cytometry - FITC Anti-CD34 antibody [4H11[APG]] (ab18227)

        Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC (ab18227). Total viable cells were used for analysis.

        ab18227 was used at a recommended dilution of 2 μg/ml.

      文献 (11)

      发表研究结果有使用 ab18227？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab18227 被引用在 11 文献中.

      • Xu J  et al. Epigenetic mechanism of miR-26b-5p-enriched MSCs-EVs attenuates spinal cord injury. Regen Ther 25:35-48 (2024). PubMed: 38058606
      • Tork S  et al. Evaluation of the Effects of Human Bone Marrow Mesenchymal Stem Cells Conditioned Medium on Growth and Maturation of Mouse Ovarian Follicle after Vitrification. Cells Tissues Organs 211:565-576 (2022). PubMed: 34348283
      • Karakaş N & Kiliç Ü Integrin α5β1 Mediated Cellular Reorganization in Human Mesenchymal Stem Cells During Neuronal Differentiation. In Vivo 35:2127-2134 (2021). PubMed: 34182488
      • Karakas N  et al. Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity. PLoS One 15:e0228510 (2020). PubMed: 32407317
      • Chen Y  et al. MSC-Secreted Exosomal H19 Promotes Trophoblast Cell Invasion and Migration by Downregulating let-7b and Upregulating FOXO1. Mol Ther Nucleic Acids 19:1237-1249 (2020). PubMed: 32069774
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Question

      What is the IgG concentration of ab18227? I want to match the concentration of an isotype control for flow cytometry.

      Read More
      Answer

      Thank you for contacting us. The concentration of ab18227, anti-CD34, has been determined and is 50 ug/ml for the lot that you have. So, 20 ul contains 1 ug.

      Abcam also carries other products essential for Flow Cytometry such as isotype controls. If testing live cells we also recommend cell viability controls such as 7-AAD or Propidium Iodide. Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AAD Cell-Mediated Cytotoxicity Assay Kit.

      Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

      7-AAD Kit: https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html

      I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

      Read More

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