AB30356

FITC荧光Anti-CD32B + CD32A抗体[AT10]

Name: FITC Anti-CD32B + CD32A抗体[AT10] (ab30356)| Abcam中文官网
Brand: Abcam Limited
SKU: AB30356
Price: 2534 CNY
Availability: InStock

FITC Anti-CD32B + CD32A antibody [AT10]

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(4 Publications)

Anti-CD32B + CD32A antibody [AT10] conjugated to FITC - suitable for intracellular flow cytometry, ICC/IF, and reacts with human samples. Clone [AT10] is a mouse monoclonal antibody cited in 33 publications.

查看别名

CD32, FCG2, IGFR2, FCGR2B, Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b, CD32, FCG2, FCGR2A1, IGFR2, FCGR2A, Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a

2 Images

Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

ab30356 staining CD32A + CD32B in K562 cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab30356 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in K562 cells fixed with 80% methanol (5 min).

Flow Cytometry (Intracellular) - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

Overlay histogram showing K562 cells stained with ab30356 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab30356, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

不同偶联物与剂型 (4)

Unconjugated

Anti-CD32B + CD32A antibody [AT10] - BSA free
Unconjugated

Anti-CD32B + CD32A antibody [AT10]
Biotin

Biotin Anti-CD32B + CD32A antibody [AT10]
578 PE

PE Anti-CD32B + CD32A antibody [AT10]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

AT10

亚型

IgG1

轻链类型

kappa

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human

应用

ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in K562 cells fixed with 4% formaldehyde (10 min) and 80% methanol (5 min).</p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

See full target information FCGR2B

其他靶点

FCGR2A

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 22:87 PubMed26475492

2015

FcγRIIB mediates antigen-independent inhibition on human B lymphocytes through Btk and p38 MAPK.

Applications

Unspecified application

Species

Unspecified reactive species

Shiang-Jong Tzeng,Wan-Yu Li,Hui-Ying Wang

Veterinary immunology and immunopathology 61:181-93 PubMed9613433

1998

Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

I Lilliehöök,A Johannisson,L Håkansson

Blood 91:2369-80 PubMed9516136

1998

Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene.

Applications

Unspecified application

Species

Unspecified reactive species

D Metes,L K Ernst,W H Chambers,A Sulica,R B Herberman,P A Morel

Molecular immunology 28:1243-54 PubMed1835758

1991

Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.

Applications

Unspecified application

Species

Unspecified reactive species

J Greenman,A L Tutt,A J George,K A Pulford,G T Stevenson,M J Glennie

View all publications

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FITC荧光Anti-CD32B + CD32A抗体[AT10]