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FITC荧光Anti-CD24抗体[SN3] (ab30350)

Reviews (1)Q&A (3)References (17)
Flow Cytometry - FITC Anti-CD24 antibody [SN3] (ab30350)

    Key features and details

    • FITC Mouse monoclonal [SN3] to CD24
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 700 APC APC/Cy7® PE PE PerCP/Cy5.5® Unconjugated

    概述

    • 产品名称

      FITC荧光Anti-CD24抗体[SN3]
      参阅全部 CD24 一抗

    • 描述

      FITC荧光小鼠单克隆抗体[SN3] to CD24

    • 宿主

      Mouse

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Glycoproteins purified from human NALM-1 cell line

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab30350于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use 20µl for 106 cells.

    1/1 - 1/10. Use 10µl of the above dilution for 106 cells or 100ul whole blood.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    说明
    Flow Cyt
    Use 20µl for 106 cells.

    1/1 - 1/10. Use 10µl of the above dilution for 106 cells or 100ul whole blood.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    靶标

    • 功能

      Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.

    • 组织特异性

      B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.

    • 疾病相关

      Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.

    • 序列相似性

      Belongs to the CD24 family.

    • 翻译后修饰

      Extensively O-glycosylated.

    • 细胞定位

      Cell membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD 24 antibody
        • CD24 antibody
        • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
        • CD24 antigen antibody
        • CD24 molecule antibody
        • CD24_HUMAN antibody
        • CD24A antibody
        • FLJ22950 antibody
        • FLJ43543 antibody
        • GPI linked surface mucin antibody
        • Heat stable antigen antibody
        • HSA antibody
        • MGC75043 antibody
        • Nectadrin antibody
        • Signal transducer CD24 antibody
        • Small cell lung carcinoma cluster 4 antigen antibody
        see all

      图片

      • Flow Cytometry - FITC Anti-CD24 antibody [SN3] (ab30350)
        Flow Cytometry - FITC Anti-CD24 antibody [SN3] (ab30350)
        Staining of human peripheral blood granulocytes with SN3 mouse monoclonal anti-human CD24 FITC conjugated (ab30350)

      文献 (17)

      发表研究结果有使用 ab30350？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab30350 被引用在 17 文献中.

      • Garza-Treviño EN  et al. Chemosensitivity analysis and study of gene resistance on tumors and cancer stem cell isolates from patients with colorectal cancer. Mol Med Rep 24:N/A (2021). PubMed: 34396431
      • Urbaniak A  et al. Single and double modified salinomycin analogs target stem-like cells in 2D and 3D breast cancer models. Biomed Pharmacother 141:111815 (2021). PubMed: 34130123
      • Zhang J  et al. Targeted inhibition of KDM6 histone demethylases eradicates tumor-initiating cells via enhancer reprogramming in colorectal cancer. Theranostics 10:10016-10030 (2020). PubMed: 32929331
      • Feng L  et al. Identification of new cancer stem cell markers and signaling pathways in HER-2-positive breast cancer by transcriptome sequencing. Int J Oncol 55:1003-1018 (2019). PubMed: 31545416
      • Huang N  et al. Neuroprotective effect of tanshinone IIA-incubated mesenchymal stem cells on Aß25-35-induced neuroinflammation. Behav Brain Res 365:48-55 (2019). PubMed: 30831140
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-4 of 4 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-CD24 antibody [SN3] (FITC)

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Pancreatic adenocarcinoma)
      Specification
      Pancreatic adenocarcinoma
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. Jennifer Dembinski

      Verified customer

      提交于 Nov 30 2009

      Question

                          Hi,   Our customer would like to know if the following antibodies are of IgG1, kappa  or lambda light chains (Information on datasheet only stated Isotypes IgG1/ IgM/ IgG2a)?   Item                                      Format                 Cat # Anti-CD200 antibody      unconjugated    ab17225 Anti-CD146 antibody      PE                           ab78488 Anti-CD34 antibody         unconjugated    ab6330 Anti-CD71 antibody         unconjugated    ab117720 Anti-CD24 antibody         FITC                       ab30350 Anti-CD73 antibody         unconjugated    ab81720                                                 PE                           ab106697 Anti-CD105 antibody      PE                           ab91238                                                 Unconjugated   ab11414 Anti-Cd45 antibody         PE                           ab90960 Anti-STRO1 antibody      unconjugated    ab102969 Anti-CD90 antibody         unconjugated    ab23894                                                 FITC                       ab11155 Anti-CD14 antibody         FITC                       ab86896               Anti-cytokeratin 15         unconjugated    ab1385 IgG2a Isotype control     FITC                       ab81197   Looking forward to your soonest reply. Thank you.   Best regards, Penny Hor BITA LIFESCIENCE SDN. BHD. C-23-05, Dataran 32, No. 2, Jalan 19/1, 46300 Petaling Jaya, Selangor Darul Ehsan, Malaysia t +603 7960 8286    e info@bitalifesc.com.my f +603 7960 8262     

      Read More
      Answer

      Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not been determined. In these instances, it is probably that the light chain sub-class is kappa, given that murine serum immunoglobulins are 95% kappa and 5% lambda, as discussed in the reference at the following link: http://www.ncbi.nlm.nih.gov/pubmed/3116408 Mol Immunol. 1987 Jul;24(7):751-7. PMID:3116408 Of the antibodies your customer has enquired about, The light chain sub-class has been determined for three: ab90960, ab86896, and ab1385. All are kappa light chain. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      I have used ab30350 in the past and it worked well in ICC. The current lot, GR30681-1 does not stain cell as expected. The previous lot stained the plasma membrane but the current lot stains the nucleus, which is not where CD24 is expressed. I am staining the same samples as before.

      Read More
      Answer

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab30350 with the order number 946525. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

      Read More

      Question

      We are interested in doing some double immunofluorescent staining of cells for CD44 and CD24. As they are both membrane located we need to go the Fluorescent route. The cells are human and fresh from biopsy samples. There is also a possibility of applying this to FFPE tissue. Can you advise on the best combination of these two Ab's to use?

      Read More
      Answer

      Thank you for your enquiry. We do not have a CD44 antibody that is directly flurophore labelled that has been shown to work by BOTH IHC-Fr and IHC-P. However, we do have the following unconjugated antibody shown to work by both applications: ab23844 CD44 antibody [MAC329] Rat Monoclonal FACS, IHC-Fr, IHC-P, IP. We have the follwing antibodies that are fluorphore conjugated but only shown to work in IHC-Fr: ab19618 CD44 antibody [IM7] Phycoerythrin conjugated Rat Monoclonal FACS, IHC-Fr, IP. ab19622 CD44 antibody [IM7] FITC conjugated Rat Monoclonal FACS, IHC-Fr, IP. With respect to CD24; again we do not have an antibody that has been shown to work by both IHC-P and IHC-Fr. However, we do have directly conjugated antibodies that have been shown to work by IHC-Fr. They are as follows: ab25657 CD24 antibody [91] FITC conjugated Rat Monoclonal FACS, IHC-Fr, IP ab25281 CD24 antibody [91] PE/Cy5 conjugated Rat Monoclonal FACS, IHC-Fr, IP ab25646 CD24 antibody [91] Phycoerythrin conjugated Rat Monoclonal FACS, IHC-Fr, IP I consider your best option using a combination of the rat monoclonal antibodies according to the wavelengths that you can detect with your fluorescence microscope. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

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