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FITC荧光Anti-CD177抗体[MEM-166] (ab26013)

Submit a review Q&A (2)References (4)
Flow Cytometry - FITC Anti-CD177 antibody [MEM-166] (ab26013)

    Key features and details

    • FITC Mouse monoclonal [MEM-166] to CD177
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    概述

    • 产品名称

      FITC荧光Anti-CD177抗体[MEM-166]
      参阅全部 CD177 一抗

    • 描述

      FITC荧光小鼠单克隆抗体[MEM-166] to CD177

    • 宿主

      Mouse

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Human
      预测可用于: Non human primates

    • 免疫原

      Tissue, cells or virus corresponding to Human CD177. Human granulocytes

    • 常规说明

      The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      pH: 7.4
      Preservative: 0.097% Sodium azide
      Constituents: PBS, 0.2% BSA
    • Concentration information loading...

    • 纯度

      Size exclusion

    • 纯化说明

      Purity >95% by SDS-PAGE.

    • 克隆

      单克隆

    • 克隆编号

      MEM-166

    • 同种型

      IgG1

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab26013于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use 20µl for 106 cells.

    or 100 µl of whole blood. (The antibody should be used undiluted with the samples).

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    说明
    Flow Cyt
    Use 20µl for 106 cells.

    or 100 µl of whole blood. (The antibody should be used undiluted with the samples).

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    靶标

    • 组织特异性

      Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).

    • 序列相似性

      Contains 2 UPAR/Ly6 domains.

    • 翻译后修饰

      N-glycosylated.
      A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).

    • 细胞定位

      Cell membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD177 antibody
        • CD177 antigen antibody
        • CD177 molecule antibody
        • CD177_HUMAN antibody
        • HNA-2a antibody
        • HNA2A antibody
        • Human neutrophil alloantigen 2a antibody
        • NB1 antibody
        • NB1 glycoprotein antibody
        • NB1 GP antibody
        • Polycythemia rubra vera 1 antibody
        • Polycythemia rubra vera protein 1 antibody
        • PRV-1 antibody
        • PRV1 antibody
        see all

      图片

      • Flow Cytometry - FITC Anti-CD177 antibody [MEM-166] (ab26013)
        Flow Cytometry - FITC Anti-CD177 antibody [MEM-166] (ab26013)

        Surface staining of human peripheral blood cells with anti-CD177.

        ab26013 was used at a recommended concentration of 1 μg/mL.

      文献 (4)

      发表研究结果有使用 ab26013？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab26013 被引用在 4 文献中.

      • Gogri H  et al. Phenotyping and Genotyping of HNA: Prevalence, Risk of Alloimmunization, and HNA Incompatibilities in Indians. Transfus Med Hemother 50:30-38 (2023). PubMed: 36818775
      • Liu H  et al. Proteinase 3 expression on the neutrophils of patients with paroxysmal nocturnal hemoglobinuria. Exp Ther Med 15:2525-2532 (2018). PubMed: 29467851
      • Herrmann IK  et al. Differentiating sepsis from non-infectious systemic inflammation based on microvesicle-bacteria aggregation. Nanoscale 7:13511-20 (2015). PubMed: 26201870
      • Hu N  et al. Decreased CXCR1 and CXCR2 expression on neutrophils in anti-neutrophil cytoplasmic autoantibody-associated vasculitides potentially increases neutrophil adhesion and impairs migration. Arthritis Res Ther 13:R201 (2011). Flow Cyt ; Human . PubMed: 22152684

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Question

      Phone call: Customer has kindly requested the information about dilution of use in flow cytometry.

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      Answer

      Thank you very much for your phone call! I can confirm that the total volume of the product is 2ml which means the product should be used undiluted in Flow Cytometry. I hope this information will be helpful. Please do not hesitate to contact me if you have any question.

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      Question

      Does this antibody react with monocytes; it has reactivity with granulocytes.

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      Answer

      Thank you for your enquiry. Further to correspondence with the source of this enquiry I can tell you that whilst the immunogen of ab26013 was isolated human granulocytes the expression of CD177 has been described in monocytes. I would therefore predict that this antibody would recognise these cells. For example the expression of CD177 on monocytes has been doucmented during pregnancy and in certain diseases: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dop t=Abstract&list_uids=15043575&query_hl=8&itool=pubmed_docsum I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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