AB26013

FITC Anti-CD177 抗体 [MEM-166]

FITC Anti-CD177 antibody [MEM-166]

Be the first to review this product! Submit a review

(4 Publications)

Mouse Monoclonal CD177 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing CD177 protein.

查看别名

CD177, NB1, PRV1, UNQ595/PRO1181, CD177 antigen, Human neutrophil alloantigen 2a, NB1 glycoprotein, Polycythemia rubra vera protein 1, HNA-2a, NB1 GP, PRV-1

1 Images

Flow Cytometry - FITC Anti-CD177 antibody [MEM-166] (AB26013)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD177 antibody [MEM-166] (AB26013)

Surface staining of human peripheral blood cells with anti-CD177.

ab26013 was used at a recommended concentration of 1 μg/mL.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MEM-166

亚型

IgG1

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human

应用

Flow Cyt

applications

免疫原

Cell preparation containing CD177 protein. The exact immunogen used to generate this antibody is proprietary information.

Q8N6Q3

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>or 100 μl of whole blood. (The antibody should be used undiluted with the samples).</p><p><a href='/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

style

left-column

产品详情

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Size-exclusion chromatography

纯化说明

Purity >95% by SDS-PAGE.

存储溶液

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD177 also known as neutrophil antigen NB1 is a glycoprotein with an approximate mass of 58 kDa. It is primarily expressed on the surface of neutrophils a type of white blood cell involved in immune response. The expression of CD177 can vary among individuals where some people might have a higher or lower presence on their neutrophils. CD177 has been identified to play a role in cell adhesion and signaling functions contributing to various cellular processes within the immune system.

Biological function summary

CD177 functions by mediating interactions between neutrophils and endothelial cells during inflammation. This protein is not known to be part of a multi-protein complex but it plays a significant role in facilitating neutrophil transmigration from blood vessels to sites of tissue damage or infection. This process supports the body's defense mechanisms by enabling neutrophils to quickly respond to external threats and participate in the inflammatory response.

Pathways

The involvement of CD177 in chemotactic activity and cell migration highlights its contribution to both the immune response and inflammation pathways. Its interaction with proteins such as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) enhances neutrophil transmigration across the endothelium. CD177 acts within these pathways to ensure a precise and rapid movement of neutrophils which is essential for an effective immune response.

CD177 levels have associations with conditions like polycythemia vera and anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. In polycythemia vera CD177 can affect the proliferation of blood cells due to its role in neutrophil function. In ANCA-associated vasculitis CD177's interaction with proteins such as PR3 (Proteinase 3) on the surface of neutrophils could contribute to the pathophysiology of the disease leading to inappropriate activation of the immune system and subsequent tissue damage.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed : 21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed : 28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed : 17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed : 23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed : 17244676, PubMed : 18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed : 23202369).

See full target information CD177

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie 50:30-38 PubMed36818775

2023

Phenotyping and Genotyping of HNA: Prevalence, Risk of Alloimmunization, and HNA Incompatibilities in Indians.

Applications

Unspecified application

Species

Unspecified reactive species

Harita Gogri,Meghana Parihar,Swati Kulkarni,Manisha Madkaikar,Jayashree Sharma,Ajit Gorakshakar

Experimental and therapeutic medicine 15:2525-2532 PubMed29467851

2018

Proteinase 3 expression on the neutrophils of patients with paroxysmal nocturnal hemoglobinuria.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Liu,Yi Liu,Yi Li,Zhaoyun Liu,Liyan Li,Shaoxue Ding,Yihao Wang,Tian Zhang,Lijuan Li,Zonghong Shao,Rong Fu

Nanoscale 7:13511-20 PubMed26201870

2015

Differentiating sepsis from non-infectious systemic inflammation based on microvesicle-bacteria aggregation.

Applications

Unspecified application

Species

Unspecified reactive species

I K Herrmann,S Bertazzo,D J P O'Callaghan,A A Schlegel,C Kallepitis,D B Antcliffe,A C Gordon,M M Stevens

Arthritis research & therapy 13:R201 PubMed22152684

2011

Decreased CXCR1 and CXCR2 expression on neutrophils in anti-neutrophil cytoplasmic autoantibody-associated vasculitides potentially increases neutrophil adhesion and impairs migration.

Applications

Flow Cyt

Species

Human

Nan Hu,Johanna Westra,Abraham Rutgers,Berber Doornbos-Van der Meer,Minke G Huitema,Coen A Stegeman,Wayel H Abdulahad,Simon C Satchell,Peter W Mathieson,Peter Heeringa,Cees G M Kallenberg

View all publications

style

left-column

style

left-column

style

right-column