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FITC荧光Anti-CD15抗体[MEM-158] (ab18272)

Reviews (2)Q&A (1)

Key features and details

  • FITC Mouse monoclonal [MEM-158] to CD15
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgM

Conjugates logo Related conjugates and formulations

APC Biotin PerCP/Cy5.5® Unconjugated

概述

  • 产品名称

    FITC荧光Anti-CD15抗体[MEM-158]
    参阅全部 CD15 一抗

  • 描述

    FITC荧光小鼠单克隆抗体[MEM-158] to CD15

  • 宿主

    Mouse

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

  • 经测试应用

    适用于: Flow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human CD15. Human granulocytes

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab18272于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use at an assay dependent concentration.

Use 200 µl/ml whole blood.

ab91546 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
说明
Flow Cyt
Use at an assay dependent concentration.

Use 200 µl/ml whole blood.

ab91546 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.

  • 细胞定位

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.

  • 别名

    • 3 fucosyl N acetyl lactosamine antibody
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

文献 (0)

发表研究结果有使用 ab18272？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab18272 尚未被引用在任何文献中。

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for FITC Anti-CD15 antibody [MEM-158]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Granulocytes)
Permeabilization
No
Specification
Granulocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 26 2021

Immunohistochemistry (Frozen sections) abreview for Anti-CD15 antibody [MEM-158] (FITC)

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Spleen)
Permeabilization
No
Specification
Spleen
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 23 2020

Question

Does this antibody react against the ssca1 antigen in addition to the Lewis x antigen?

Read More
Answer

Thank you for your enquiry. I hope that you receive our chromatin marketing material shortly. I mailed it to you yesterday. I have received the following response from the source of this antibody. "The CD15 antigen is expressed on immature bone marrow cells and leukemic cells of the myelo-monocytic lineage and sometimes on lymphocytic leukemia cells. ab18272 reacts with granulocytes and has virtually no reactivity against monocytes. I had tried to find all posssible informations in HLDA Workshop VI (WS code of ab18272 A053). I did not find any info about reactivity against ssca1 antigen. Thanks for understanding, I hope that this info will help you". I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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